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Development of SSR markers for the phylogenetic analysis of almond trees from China and the Mediterranean region

文献类型： 外文期刊

作者： Xu, Y 1 ; Ma, RC 2 ; Xie, H 2 ; Liu, JT 2 ; Cao, MQ 2 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China; Western Erdos Nat Conservat Zone China, Erdos 017000, Inner Mongolia, Peoples R China

关键词： expressed sequence tags (ESTs);microsatellites (SSRs);almond;P. communis Fritsch

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN： 0831-2796

年卷期： 2004 年 47 卷 6 期

页码：

收录情况： SCI

摘要： Expressed sequence tag (EST) derived simple sequence repeats (SSRs, microsatellites) were screened and identified from 3863 almond and 10 185 peach EST sequences, and the spectra of SSRs in the non-redundant EST sequences were investigated after sequence assembly. One hundred seventy-eight (12.07%) almond SSRs and 497 (9.97%) peach SSRs were detected. The EST SSR occurs every 4.97 kb in almond ESTs and 6.57 kb in peach, and SSRs with di- and trinucleotide repeat motifs are the most abundant in both almond and peach ESTs. Twenty one EST SSRs were thereafter, developed and used together with 7 genomic SSRs, to study the genetic relationship among 36 almond (P. communis Fritsch.) cultivars from China and the Mediterranean area, as well as 8 accessions of other related species from the genus Prunus. Both EST derived and genomic SSR markers showed high cross-species transferability in the genus. Out of the 112 polymorphic alleles detected in the 36 cultivated almonds, 28 are specific to Chinese cultivars and 25 to the others. The 44 accessions were clustered into 4 groups in the phylogenetic tree and the 36 almond cultivars formed two distinct subgroups, one containing only Chinese cultivars and one of unknown origin and the other only those originating from the Mediterranean area, indicating that Chinese almond cultivars have a distinct evolutionary history from the Mediterranean almond. Our preliminary results indicated that common almond was more closely related to peach (P. persica (L.) Batsch.) than to the four wild species of almond, (P mongolica Maxim., P. ledebouriana Schleche, P. tangutica Batal., and P. triloba Lindl.). The implications of these SSR markers for evolutionary analysis and molecular mapping of Prunus species are discussed.

  • 相关文献

[1]Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils. Jiang, YQ,Ma, RC. 2003

[2]Pollen Tube Behavior in Inter-specific Cross between Prunus Armeniaca L. and Prunus Amygdalus Batsch.. Sun Haoyuan,Wang Yuzhu,Yang Li,Cai Shengwen. 2011

[3]IDENTIFICATION AND CHARACTERIZATION OF 43 NOVEL POLYMORPHIC EST-SSR MARKERS FOR ARUM LILY, ZANTEDESCHIA AETHIOPICA (ARACEAE). Wei, Zun-Zheng,Xiong, Min,Wang, Xian,Zhou, Di,Luo, Lai-Bo,Zhang, Hua-Li. 2012

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