文献类型： 外文期刊

作者： Zhang, Huilong ¹ ; Liu, Zhen ³ ; Hu, Aishuang ¹ ; Wu, Haiwen ¹ ; Zhu, Jianfeng ¹ ; Wang, Fengzhi ³ ; Cao, Pingping ³ ; Yang, Xiuyan ¹ ; Zhang, Huaxin ¹ ;

作者机构： 1.Chinese Acad Forestry, Inst Ecol Protect & Restorat, Beijing 100091, Peoples R China

2.Chinese Acad Forestry Yellow River Delta, Comprehens Expt Ctr, Dongying 257000, Peoples R China

3.Hebei Key Lab Crop Salt Alkali Stress Tolerance E, Cangzhou 061001, Peoples R China

4.Acad Agr & Forestry Sci, Cangzhou 061001, Peoples R China

5.Hebei Acad Agr & Forestry Sci, Inst Coastal Agr, Tangshan 063299, Peoples R China

关键词： full-length transcriptome analysis; Nitraria sibirica; H+-PPase; salt stress

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Background: Nitraria sibirica Pall. is one of the pioneer tree species in saline-alkali areas due to its extreme salt tolerance. However, the lack of information on its genome limits the further exploration of the molecular mechanisms in N. sibirica under salt stress. Methods: In this study, we used single-molecule real-time (SMRT) technology based on the PacBio Iso-Seq platform to obtain transcriptome data from N. sibirica under salt treatment for the first time, which is helpful for our in-depth analysis of the salt tolerance and molecular characteristics of N. sibirica. Results: Our results suggested that a total of 234,508 circular consensus sequences (CCSs) with a mean read length of 2121 bp were obtained from the 19.26 Gb raw data. Furthermore, based on transcript cluster analysis, 93,713 consensus isoforms were obtained, including 92,116 high-quality isoforms. After removing redundant sequences, 49,240 non-redundant transcripts were obtained from high-quality isoforms. A total of 37,261 SSRs, 1816 LncRNAs and 47,314 CDSs, of which 40,160 carried complete ORFs, were obtained. Based on our transcriptome data, we also analyzed the coding genes of H+-PPase, and the results of both bioinformatics and functional analyses indicated that the gene prediction via full-length transcripts obtained by SMRT technology is reliable and effective. In summary, our research data obtained by SMRT technology provides more reliable and accurate information for the further analysis of the regulatory network and molecular mechanism of N. sibirica under salt stress.