Coordinated transcriptional regulation of carbohydrate-related pathways contributes to the difference of starch accumulation between starchy cassava and sugary cassava
文献类型： 外文期刊
作者： Ding, Zehong 1 ; Fu, Lili 1 ; Chen, Ganlu 1 ; Yan, Yan 1 ; Tie, Weiwei 1 ; Meng, Xianwei 1 ; Yang, Jinghao 1 ; Qiu, Xianjin 4 ; Zhang, Jiaming 1 ; Hu, Wei 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Sanya Res Inst,Natl Key Lab Trop Crop Breeding, Inst Trop Biosci & Biotechnol,Key Lab Biol & Genet, Haikou, Peoples R China
2.Hainan Seed Ind Lab, Sanya, Peoples R China
3.Chinese Acad Trop Agr Sci, Hainan Inst Trop Agr Resources, Hainan Key Lab Protect & Utilizat Trop Bioresource, Haikou, Peoples R China
4.Yangtze Univ, Coll Agr, Jingzhou 434022, Hubei, Peoples R China
关键词： Starchy cassava; Sugary cassava; Starch accumulation; Metabolome; Transcriptome; Coordinated transcriptional regulation; Coexpression network
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：12.5； 五年影响因子：11.9 ）
ISSN： 0144-8617
年卷期： 2025 年 354 卷
页码：
收录情况： SCI
摘要： Cassava is a significant starchy root crop providing staple foods for millions of people globally. However, the metabolic differences and regulatory mechanisms underlying starch accumulation remain largely elusive in cassava. In this work, scanning electron microscopy, metabolome, and transcriptome were analyzed in parallel between starchy cassava (SC205) and sugary cassava (SM) during storage root (SR) development. Many carbohydrate-related metabolites (including fructose-6-phosphate and glucose-6-phosphate) were differentially accumulated between SC205 and SM. Further analysis unveiled coordinated metabolomic and transcriptomic changes in sucrose and starch metabolism, glycolysis and TCA cycle, lipid metabolism, and lignin and flavonoid biosynthesis during SR development, but with distinct abundance patterns between SC205 and SM. Specifically, bZIP2 could directly bind to the promoters of APL1, ISA1, and GBSS1 and promote their expression levels in SC205, but these interactions were absent in SM. Transient silencing of APL1, ISA1, or GBSS1 resulted in significant decreases in starch contents. Transient silencing of bZIP2 suppressed the expression of APL1, ISA1, and GBSS1, and accordingly, decreased the starch contents. These results suggest a crucial contribution of coordinated regulation of carbohydrate-related pathways/genes to the difference of starch accumulation between starchy cassava and sugary cassava, providing useful information for starch improvement in the future.
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