文献类型： 外文期刊

作者： Chen, Min ¹ ; Guo, Hao-Ming ¹ ; Di, Shan-Shan ² ; Zhao, Yue ¹ ; Zhou, Dong-Dong ¹ ; Cao, Yi-Wen ¹ ; Tian, Pei ² ; Yang, Zhong-Hua ¹ ; Zhao, Hui-Yu ² ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Dept Plant Protect, Wuhan, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, key Lab Detect Pesticide Residues & Control Zhejia, Hangzhou, Peoples R China

3.Agr Minist Key Lab Pesticide Residue Detect, Hangzhou, Peoples R China

4.Huazhong Agr Univ, Coll Plant Sci & Technol, Dept Plant Protect, Wuhan 430070, Peoples R China

5.Agr Minist Key Lab Pesticide Residue Detect, Hangzhou 310021, Peoples R China

关键词： enantiomeric effect; high-throughput sequencing; microorganism; paclobutrazol

期刊名称：CHIRALITY （ 影响因子：2.0； 五年影响因子：1.9 ）

ISSN： 0899-0042

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Studies on the differences between chiral pesticide enantiomers have caused widespread concern in the last decade. In the current work, the selective behaviors and different biological activities of paclobutrazol enantiomers during Chinese cabbage pickling process were evaluated. Results of degradation kinetics indicated that when paclobutrazol reside in raw material (Chinese cabbage) and was introduced into the pickling process, the degradation rates of the two paclobutrazol enantiomers were significantly different, the half-lives of (2R, 3R)-paclobutrazol (R-paclobutrazol) and (2S, 3S)-paclobutrazol (S-paclobutrazol) were 18.24 and 6.19 d, respectively. Besides, the conversion between the two enantiomers could also be observed, and the conversion rate of R-paclobutrazol to S-paclobutrazol was slower than that of reverse process. In addition, from the analysis of 16S rRNA and ITS sequencing, we inferred that the degradation of paclobutrazol was probably due to the presence of Pseudomonas and Serratia. Moreover, there has a significant difference in biological activity between R-paclobutrazol and S-paclobutrazol and shown an obviously enantiomeric effects on microbial community composition of pickling system. Besides, the analysis of microbial community displayed R-paclobutrazol might inhibit the growth of Erwinia (a sort of plant pathogens). Results from this study served to enhance our understanding of chiral pesticide residues on food safety and the potential risks to human health.