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Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage

文献类型： 外文期刊

作者： Gu, AiXia 1 ; Meng, Chuan; Chen, YueQi 1 ; Wei, Lai 1 ; Dong, Hui; Lu, Yin 1 ; Wang, YanHua 1 ; Chen, XuePing 1 ; Zhao 1 ;

作者机构： 1.Hebei Agr Univ, Key Lab Vegetable Germplasm Innovat & Utilizat He, Collaborat Innovat Ctr Vegetable Ind Hebei, Coll Hort, Baoding, Peoples R China

2.Hebei Agr Univ, Key Lab Vegetable Germplasm Innovat & Utilizat He, Collaborat Innovat Ctr Vegetable Ind Hebei, Coll Hort, Baoding, Peopl

关键词： Chinese cabbage;heading type;plant hormone;BrGH3.12;BrABF1;Seq-BSA;RNA-Seq

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： In Chinese cabbage, heading type is a key agricultural trait of significant economic importance. Using a natural microspore-derived doubled haploid plant, we generated self-crossed progeny with overlapping or outward curling head morphotypes. Sequencing-based bulked segregant analysis (Seq-BSA) revealed a candidate region of 0.52 Mb (A06: 1,824,886 similar to 2,347,097 bp) containing genes enriched for plant hormone signal transduction. RNA Sequencing (RNA-Seq) analysis supported the hormone pathway enrichment leading to the identification of two key candidate genes, BrGH3.12 and BrABF1. The regulated homologous genes and the relationship between genes in this pathway were also revealed. Expression of BrGH3.12 varied significantly in the apical portion of the leaf, consistent with the morphological differences between overlapping and outward curling leaves. Transcript levels of BrABF1 in the top, middle and basal segments of the leaf were significantly different between the two types. The two morphotypes contained different concentrations of IAA in the apical portion of their leaves while levels of ABA differed significantly between plant types in the top, middle, and basal leaf segments. Results from Seq-BSA, RNA-Seq and metabolite analyses all support a role for IAA and ABA in heading type formation. These findings increase our understanding of the molecular basis for pattern formation of the leafy head in Chinese cabbage and will contribute to future work developing more desirable leafy head patterns.

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