您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transcriptome and Gene Expression Analysis of Cylas formicarius (Coleoptera: Brentidae) During Different Development Stages

文献类型： 外文期刊

作者： Ma, Juan 1 ; Wang, Rongyan 1 ; Li, Xiuhua 1 ; Gao, Bo 1 ; Chen, Shulong 1 ;

作者机构： 1.Minist Agr, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov, Inst Plant Protect,Hebei Acad Agr & Forestry Sci, 437 Dongguan St, Baoding 071000, Peoples R China

关键词： different stages;differentially expressed genes;sweet potato weevil;transcriptome

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2016 年 16 卷

页码：

收录情况： SCI

摘要： The sweet potato weevil, Cylas formicarius (F.) (Coleoptera: Brentidae), is an important pest of sweet potato worldwide. However, there is limited knowledge on the molecular mechanisms underlying growth and differentiation of C. formicarius. The transcriptomes of the eggs, second instar larvae, third instar larvae (L3), pupae, females, and males of C. formicarius were sequenced using Illumina sequencing technology for obtaining global insights into developing transcriptome characteristics and elucidating the relative functional genes. A total of 54,255,544 high-quality reads were produced, trimmed, and de novo assembled into 115,281 contigs. 61,686 unigenes were obtained, with an average length of 1,009 nt. Among these unigenes, 17,348 were annotated into 59 Gene Ontology (GO) terms and 12,660 were assigned to 25 Cluster of Orthologous Groups classes, whereas 24,796 unigenes were mapped to 258 pathways. Differentially expressed unigenes between various developmental stages of C. formicarius were detected. Higher numbers of differentially expressed genes (DEGs) were recorded in the eggs versus L3 and eggs versus male samples (2,141 and 2,058 unigenes, respectively) than the others. Genes preferentially expressed in each stage were also identified. GO and pathway-based enrichment analysis were used to further investigate the functions of the DEGs. In addition, the expression profiles of ten DEGs were validated by quantitative real-time PCR. The transcriptome profiles presented in this study and these DEGs detected by comparative analysis of different developed stages of C. formicarius will facilitate the understanding of the molecular mechanism of various living process and will contribute to further genome-wide research.

  • 相关文献

[1]A deep sequencing analysis of transcriptomes and the development of EST-SSR markers in mungbean (Vigna radiata). Liu, Changyou,Fan, Baojie,Cao, Zhimin,Su, Qiuzhu,Wang, Yan,Zhang, Zhixiao,Tian, Jing,Wu, Jing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台