河北省农林科学院机构知识库

Molecular identification of omega-secalin gene expression activity in a wheat 1B/1R translocation cultivar

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文献类型：外文期刊

作者： Chai Jian-fang ¹ ; Zhang Cui-mian ¹ ; Ma Xiu-ying ¹ ; Wang Hai-bo ¹ ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Plant Genet Engn Ctr Hebei Prov, Shijiazhuang 050051, Peoples R China

关键词： Triticum aestivum;processing quality;storage proteins;prokaryotic expression;LC-MS/MS

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （影响因子：2.848；五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 12 期

页码：

收录情况： SCI

摘要： omega-Secalin was an important factor influencing processing quality of wheat 1BL/1RS translocations. On the basis of four omega-secalin gene sequences cloned from Lankao 906 (a wheat cultivar with 1BL/1RS translocation) with putative transcription activity, a pair of primers with suitable restriction endonucleases added at their 5' ends were designed to amplify the mature protein-coding regions of the four genes. After digestion with restriction endonucleases, the amplified products were ligated into the prokaryotic expression vector pET30a(+). The prokaryotically expressed recombinant proteins and gliadin isolated from the Lankao 906 seed were analyzed on the same acid polyacrylamide gel electrophoresis. All four prokaryotically expressed secalin bands had corresponding seed-expressed gliadin bands. The four corresponding gliadin bands were confirmed to be the expression products of the four w-secalin genes by liquid chromatography tandem mass spectrometry (LC-MS/MS). This conclusion was further confirmed because the four omega-secalin bands could be detected in all 14 wheat 1BL/1RS translocation cultivars used in the study, although there was some interference for the detection of one w-secalin band from nearby wheat gliadin bands. The sequence information of w-secalin genes with expression activity will be helpful for improving the processing quality of wheat with 1BL/1RS translocations by using RNA interference method to silence the expression of the omega-secalin genes.

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Molecular identification of omega-secalin gene expression activity in a wheat 1B/1R translocation cultivar

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Molecular identification of omega-secalin gene expression activity in a wheat 1B/1R translocation cultivar

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