MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica
文献类型： 外文期刊
作者： Li Po 1 ; Gong Xiao-dong 1 ; Jia Hui 1 ; Fan Yong-shan 1 ; Zhang Yun-feng 1 ; Han Jian-min 1 ; Gu Shou-qin 1 ; Dong Jin-g 1 ;
作者机构： 1.Agr Univ Hebei, Mycotoxin & Mol Plant Pathol Lab, Baoding 071000, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Peoples R China
3.Tangshan Normal Univ, Tangshan 063000, Peoples R China
关键词： Setosphaeria turcica;MAPK;conidiogenesis;HT-toxin;pathogenicity
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2016 年 15 卷 12 期
页码：
收录情况： SCI
摘要： The mitogen-activated protein kinase (MAPK), a key signal transduction component in the MAPK cascade pathway, regulates a variety of physiological activities in eukaryotes. However, little is known of the role MAPK plays in phytopathogenic fungi. In this research, we cloned the MAPK gene STK1 from the northern corn leaf blight pathogen Setosphaeria turcica and found that the gene shared high homology with the high osmolality glycerol (HOG) MAPK gene HOG1 of Saccharomyces cerevisiae. In addition, gene knockout technology was employed to investigate the function of STK1. Gene knockout mutants (KOs) were found to have altered hyphae morphology and no conidiogenesis, though they did show similar radial growth rate compared to the wild-type strain (WT). Furthermore, microscope observations indicated that STK1 KOs did not form normal appressoria at 48 h post-inoculation on a hydrophobic surface. STK1 KOs had reduced virulence, a significantly altered Helminthosporium turcicum (HT)-toxin composition, and diminished pathogenicity on the leaves of susceptible inbred corn OH43. Mycelium morphology appeared to be significantly swollen and the radial growth rates of STK1 KOs declined in comparison with WT under high osmotic stress. These results suggested that STK1 affects the hyphae development, conidiogenesis, and pathogenicity of S. turcica by regulating appressorium development and HT-toxin biosynthesis. Moreover, the gene appears to be involved in the hypertonic stress response in S. turcica.
- 相关文献
作者其他论文 更多>>
-
Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica, Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation, Invasive Growth, and Mating Behavior
作者：Gu Shou-qin;Yang Yang;Zhang Chang-zhi;Fan Yu;Zhang Xiao-yu;Tian Lan;Hao Zhi-min;Cao Zhi-yan;Gong Xiao-dong;Han Jian-min;Dong Jin-gao;Li Po;Fan Yong-shan
关键词：Setosphaeria turcica;MAPK;filamentation;invasive growth;mating behavior
