文献类型： 外文期刊

作者： Yang, Nina ¹ ; Sun, Yuhan ¹ ; Wang, Yaru ¹ ; Long, Cui ¹ ; Li, Yingyue ¹ ; Li, Yun ¹ ;

作者机构： 1.Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Natl Engn Lab Tree Breeding, Minist Educ,Coll Biol Sci & Technol, Beijing, Peoples R China

2.Hebei Acad Agr & Forestry Sc

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 10 期

页码：

收录情况： SCI

摘要： Colchicine treatment of G. biloba microsporocytes results in a low mutation rate in the diploid (2n) male gamete. The mutation rate is significantly lower as compared to other tree species and impedes the breeding of new economic varieties. Proteomic analysis was done to identify the proteins that influence the process of 2n gamete formation in G. biloba. The microsporangia of G. biloba were treated with colchicine solution for 48 h and the proteins were analyzed using 2-D gel electrophoresis and compared to protein profiles of untreated microsporangia. A total of 66 proteins showed difference in expression levels. Twenty-seven of these proteins were identified by mass spectrometry. Among the 27 proteins, 14 were found to be up-regulated and the rest 13 were down-regulated. The identified proteins belonged to five different functional classes: ATP generation, transport and carbohydrate metabolism; protein metabolism; ROS scavenging and detoxifying enzymes; cell wall remodeling and metabolism; transcription, cell cycle and signal transduction. The identification of these differentially expressed proteins and their function could help in analysing the mechanism of lower mutation rate of diploid male gamete when the microsporangium of G. biloba was induced by colchicine.