New Type of Antimicrobial Protein Produced by the Plant Pathogen Clavibacter michiganensis subsp michiganensis
文献类型： 外文期刊
作者： Liu, Zhanliang 1 ; Ma, Ping 2 ; Holtsmark, Ingrid 1 ; Skaugen, Morten 1 ; Eijsink, Vincent G. H. 1 ; Brurberg, May 1 ;
作者机构： 1.Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, As, Norway
2.Hebei Acad Agr & Forestry Sci, Integrated Pest Management Ctr Hebei Provence, Inst Plant Protect, Baoding, Peoples R China
3.Norwegian Inst Agr & Environm Res, Plant Hlth & Plant Protect Div, As, Norway
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）
ISSN： 0099-2240
年卷期： 2013 年 79 卷 18 期
页码：
收录情况： SCI
摘要： It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease.
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