New Type of Antimicrobial Protein Produced by the Plant Pathogen Clavibacter michiganensis subsp michiganensis
文献类型： 外文期刊
作者： Liu, Zhanliang 1 ; Ma, Ping 2 ; Holtsmark, Ingrid 1 ; Skaugen, Morten 1 ; Eijsink, Vincent G. H. 1 ; Brurberg, May 1 ;
作者机构： 1.Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, As, Norway
2.Hebei Acad Agr & Forestry Sci, Integrated Pest Management Ctr Hebei Provence, Inst Plant Protect, Baoding, Peoples R China
3.Norwegian Inst Agr & Environm Res, Plant Hlth & Plant Protect Div, As, Norway
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）
ISSN： 0099-2240
年卷期： 2013 年 79 卷 18 期
页码：
收录情况： SCI
摘要： It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease.
- 相关文献
作者其他论文 更多>>
-
Establishment and application of quantitative detection of Bacillus velezensis HMB26553, a biocontrol agent against cotton damping-off caused by Rhizoctonia
作者：Su, Zhenhe;Liu, Gaoge;Li, Cong;Liu, Xiaomeng;Guo, Qinggang;Wang, Peipei;Dong, Lihong;Lu, Xiuyun;Zhao, Weisong;Zhang, Xiaoyun;Qu, Yuanghang;Zhang, Jiaqi;Mo, Shaojing;Li, Shezeng;Ma, Ping;Ma, Ping
关键词：Bacillus velezensis; detection; quantification; strain-specific primers; TaqMan qPCR; unique gene pB-gene0026
-
Effects of different crop rotations on the incidence of cotton Verticillium wilt and structure and function of the rhizospheric microbial community
作者：Zhao, Weisong;Li, Shezeng;Dong, Lihong;Wang, Peipei;Lu, Xiuyun;Zhang, Xiaoyun;Su, Zhenhe;Guo, Qinggang;Ma, Ping;Zhao, Weisong;Li, Shezeng;Dong, Lihong;Wang, Peipei;Lu, Xiuyun;Zhang, Xiaoyun;Su, Zhenhe;Guo, Qinggang;Ma, Ping
关键词：Crop rotation; Cotton Verticillium wilt; Rhizosphere microbes; Metagenome; Nutrients cycling
-
Quantitative detection of the Ralstonia solanacearum species complex in soil by qPCR combined with a recombinant internal control strain
作者：Chen, Wei;Zhang, Jun-Wei;Zhang, Zhi;Qiao, Xiu-Ze;Li, Shan-Kui;Asif, Muhammad;Guo, Song;Jiang, Wen-Jun;Zhang, Li-Qun;Qin, Bi-Xia;Xie, Hui-Ting;Cui, Li-Xian;Wang, Pei-Pei;Dong, Li-Hong;Guo, Qing-Gang;Ma, Ping;Xia, Zhen-Yuan;Lu, Can-Hua
关键词：Ralstonia solanacearum species complex; quantitative PCR; large-scale sequence analysis; internal sample process control
-
Colonization Ability of Bacillus subtilis NCD-2 in Different Crops and Its Effect on Rhizosphere Microorganisms
作者：Zhao, Weisong;Ban, Yiyun;Su, Zhenhe;Li, Shezeng;Liu, Xiaomeng;Guo, Qinggang;Ma, Ping
关键词：Bacillus subtilis NCD-2; colonization; growth promotion; rhizosphere microbiome
-
Screening Biocontrol Agents for Cash Crop Fusarium Wilt Based on Fusaric Acid Tolerance and Antagonistic Activity against Fusarium oxysporum
作者：Guo, Qinggang;Li, Shixin;Dong, Lihong;Su, Zhenhe;Wang, Peipei;Liu, Xiaomeng;Ma, Ping
关键词：Bacillus velezensis; biological control; Fusarium wilt; fusaric acid; tolerant
-
Functional Analyses of the Bacillus velezensis HMB26553 Genome Provide Evidence That Its Genes Are Potentially Related to the Promotion of Plant Growth and Prevention of Cotton Rhizoctonia Damping-Off
作者：Su, Zhenhe;Liu, Gaoge;Liu, Xiaomeng;Li, Shezeng;Lu, Xiuyun;Wang, Peipei;Zhao, Weisong;Zhang, Xiaoyun;Dong, Lihong;Qu, Yuanhang;Zhang, Jiaqi;Mo, Shaojing;Guo, Qinggang;Ma, Ping
关键词：Bacillus velezensis; biocontrol agent; genome mining; secondary metabolites; pathogen inhibition; cotton growth promotion
-
Effect of incorporation of broccoli residues into soil on occurrence of verticillium wilt of spring-sowing-cotton and on rhizosphere microbial communities structure and function
作者：Zhao, Weisong;Wang, Peipei;Dong, Lihong;Li, Shezeng;Lu, Xiuyun;Zhang, Xiaoyun;Su, Zhenhe;Guo, Qinggang;Ma, Ping
关键词：spring-sowing-cotton; verticillium wilt; broccoli residues; microbial community structure; function; rhizosphere
