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Phosphoinositide-specific phospholipase C9 is involved in the thermotolerance of Arabidopsis

文献类型： 外文期刊

作者： Zheng, Shu-Zhi 1 ; Liu, Yu-Liang 1 ; Li, Bing 1 ; Shang, Zhong-lin 1 ; Zhou, Ren-Gang 2 ; Sun, Da-Ye 1 ;

作者机构： 1.Hebei Normal Univ, Hebei Key Lab Mol & Cellular Biol, Coll Life Sci, Shijiazhuang 050016, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Shijiazhuang 050051, Peoples R China

关键词： AtPLC9;heat shock;signal transduction;thermotolerance;Arabidopsis

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2012 年 69 卷 4 期

页码：

收录情况： SCI

摘要： Intracellular calcium (Ca2+) increases rapidly after heat shock (HS) in the Ca2+/calmodulin (Ca2+/CaM) HS signal transduction pathway: a hypothesis proposed based on our previous findings. However, evidence for the increase in Ca2+ after HS was obtained only through physiological and pharmacological experiments; thus, direct molecular genetic evidence is needed. The role of phosphoinositide-specific phospholipase C (PI-PLC) is poorly understood in the plant response to HS. In this work, atplc9 mutant plants displayed a serious thermosensitive phenotype compared with wild-type (WT) plants after HS. Complementation of atplc9 with AtPLC9 rescued both the basal and acquired thermotolerance phenotype of the WT plants. In addition, thermotolerance was even improved in overexpressed lines. The GUS staining of AtPLC9 promoter:GUS transgenic seedlings showed that AtPLC9 expression was ubiquitous. The fluorescence distribution of the fusion protein AtPLC9 promoter:AtPLC9:GFP revealed that the subcellular localization of AtPLC9 was restricted to the plasma membrane. The results of a PLC activity assay showed a reduction in the accumulation of inositol-1,4,5-trisphosphate (IP3) in atplc9 during HS and improved IP3 generation in the overexpressed lines. Furthermore, the heat-induced increase in intracellular Ca2+ was decreased in atplc9. Accumulation of the small HS proteins HSP18.2 and HSP25.3 was downregulated in atplc9 and upregulated in the overexpressed lines after HS. Together, these results provide molecular genetic evidence showing that AtPLC9 plays a role in thermotolerance in Arabidopsis.

  • 相关文献

[1]Arabidopsis thaliana Phosphoinositide-Specific Phospholipase C Isoform 3 (AtPLC3) and AtPLC9 have an Additive Effect on Thermotolerance. Gao, Kang,Liu, Yu-Liang,Li, Bing,Sun, Da-Ye,Zheng, Shu-Zhi,Gao, Kang,Liu, Yu-Liang,Li, Bing,Sun, Da-Ye,Zheng, Shu-Zhi,Gao, Kang,Liu, Yu-Liang,Li, Bing,Sun, Da-Ye,Zheng, Shu-Zhi,Zhou, Ren-Gang.

[2]The Arabidopsis J-protein AtDjB1 facilitates thermotolerance by protecting cells against heat-induced oxidative damage. Zhou, Wei,Zhou, Ting,Li, Mi-Xin,Zhao, Chun-Lan,Jia, Ning,Wang, Xing-Xing,Sun, Yong-Zhen,Xu, Meng,Li, Bing,Zhou, Wei,Li, Guo-Liang,Zhou, Ren-Gang,Zhou, Wei. 2012

[3]A heat-activated calcium-permeable channel - Arabidopsis cyclic nucleotide-gated ion channel 6 - is involved in heat shock responses. Gao, Fei,Zhou, Rengang,Gao, Fei,Han, Xiaowei,Wu, Jianhai,Zheng, Shuzhi,Shang, Zhonglin,Sun, Daye,Li, Bing,Gao, Fei.

[4]Expression of maize heat shock transcription factor gene ZmHsf06 enhances the thermotolerance and drought-stress tolerance of transgenic Arabidopsis. Li, Hui-cong,Zhang, Hua-ning,Li, Guo-liang,Liu, Zi-hui,Zhang, Yan-min,Zhang, Hong-mei,Guo, Xiu-lin.

[5]TraeALDH7B1-5A, encoding aldehyde dehydrogenase 7 in wheat, confers improved drought tolerance in Arabidopsis. Chen, Jiamin,Wei, Bo,Li, Guoliang,Fan, Renchun,Zhong, Yongda,Wang, Xianping,Zhang, Xiangqi,Chen, Jiamin,Li, Guoliang,Li, Guoliang,Zhong, Yongda.

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