您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase

文献类型： 外文期刊

作者： Liu, Zi-Hui 1 ; Zhang, Hong-Mei 1 ; Li, Guo-Liang 1 ; Guo, Xiu-Lin 1 ; Chen, Shou-Yi 3 ; Liu, Gui-Bo 4 ; Zhang, Yan-Mi 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Shijiazhuang 050051, Peoples R China

2.Plant Genet Engn Ctr Hebei Prov, Shijiazhuang 050051, Peoples R China

3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Dry Land Farming, Hengshui 053000, Peoples R China

关键词： Medicago sativa L.;BADH;Transformation;Salt tolerance

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2011 年 178 卷 3 期

页码：

收录情况： SCI

摘要： Gene BADH was transformed into alfalfa (Medicago sativa L.) through Agrobacterium-mediated transformation method, and salt tolerance of the transgenic progenies was assessed. Among 247 plants obtained after transformation, 43 were positive by polymerase chain reaction (PCR) detection using the primers specific for BADH gene. Reverse transcription polymerase chain reaction (RT-PCR) analysis indicated that gene BADH was expressed in 42 plants at transcriptional level. Southern blotting analysis also showed that the target was integrated into Medicago genome. The transgenic plants grew vigorous in salt stress condition, whereas the wild type plants was retarded and did not survive after cradle. The relative electrical conductivity and malondialdehyde (MDA) contents in the T(1) transgenic plants were lower, but peroxidase (POD) and superoxide dismutase (SOD) activities were higher than those of the wild type plants. These results demonstrated that the expression of foreign BADH gene enhanced the salt tolerance in transgenic alfalfa.

  • 相关文献

[1]转BADH基因苜蓿T-DNA侧翼序列分析及转化事件特异性分析. 张艳敏,张红梅,相金英,郭秀林,刘子会,李国良,陈受宜. 2011

[2]外源甜菜碱醛脱氢酶(BADH)基因对小麦合成甜菜碱的影响. 丁占生,张艳敏,张光荣,温之雨,郭北海. 2003

[3]转甜菜碱醛脱氢酶(BADH)基因小麦的耐盐耐旱性. 张艳敏,郭北海,蒋春志,温之雨,丁占生,李辉,李洪杰,何锶洁,陈受宜,朱至清. 2003

[4]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[5]Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase (BADH). Guo, BH,Zhang, YM,Li, HJ,Du, LQ,Li, YX,Zhang, JS,Chen, SY,Zhu, ZQ. 2000

[6]Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction. Zhang, Yan-Min,Zhang, Hong-Mei,Liu, Zi-Hui,Guo, Xiu-Lin,Li, Hui-Cong,Li, Guo-Liang,Jiang, Chun-Zhi,Zhang, Meng-Chen.

[7]Improvement of soybean transformation via Agrobacterium tumefaciens methods involving alpha-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis. Zhang, Yan-Min,Liu, Zi-Hui,Yang, Rui-Juan,Li, Guo-Liang,Guo, Xiu-Lin,Zhang, Hua-Ning,Zhang, Hong-Mei,Di, Rui,Zhao, Qing-Song,Zhang, Meng-Chen.

[8]Expression of maize heat shock transcription factor gene ZmHsf06 enhances the thermotolerance and drought-stress tolerance of transgenic Arabidopsis. Li, Hui-cong,Zhang, Hua-ning,Li, Guo-liang,Liu, Zi-hui,Zhang, Yan-min,Zhang, Hong-mei,Guo, Xiu-lin.

[9]SnRK2 Homologs in Gossypium and GhSnRK2.6 Improved Salt Tolerance in Transgenic Upland Cotton and Arabidopsis. Su, Ying,Zhen, Junbo,Zhang, Xi,Chen, Zhiwen,Li, Le,Hua, Jinping,Wang, Yumei,Zhen, Junbo,Huang, Yi.

[10]The wheat NHX antiporter gene TaNHX2 confers salt tolerance in transgenic alfalfa by increasing the retention capacity of intracellular potassium. Zhang, Yan-Min,Zhang, Hong-Mei,Liu, Zi-Hui,Li, Hui-Cong,Guo, Xiu-Lin,Li, Guo-ang.

[11]Overexpression of an Aeluropus littoralis Parl. potassium transporter gene, AlHAK1, in cotton enhances potassium uptake and salt tolerance. Liu, J. F.,Zhang, S. L.,Tang, H. L.,Dong, L. J.,Wu, L. Z.,Liu, L. D.,Che, W. L..

[12]Field Screening and Evaluation of Salt and Cold Tolerance of Wheat Germplasm Resources. Li, X. P.,Lan, S. Q.,Zhang, Y. L.,Liu, S. E.,Wang, L. N.,Lu, L.,Rajaram, S..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台