文献类型： 外文期刊

作者： Liu Hai-tao ¹ ; Li Cai-li ² ; Zhang Yu-jing ¹ ; Li Chun-min ² ; Zhao Yong-bo ² ; Chen Dong-mei ² ; Wang Yi ¹ ; Zhang Xin- ¹ ;

作者机构： 1.China Agr Univ, Inst Hort Plants, Coll Agron & Biotechnol, Beijing 100193, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Peoples R China

3.NE Agr Univ, Coll Hort, Harbin 150030, Peoples R China

关键词： Malus domestica Borkh.;Botryosphaeria dothidea;inheritance;microsatellite;AFLP

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Apple bot canker [Botryosphaeria dothidea (Moug.) Ces. et de Not.] is distributed worldwide, resulting in a serious crop loss every year in apple (Malus domestica Borkh.) production. The resistance of each seedling derived from a hybrid population (JonathanxGolden Delicious) was evaluated by disease index either from natural infection in the field or from inoculation with five isolates of B. dothidea, Ls1, Lw023, Lw048, Mx1, and Zz26. The inheritance of the resistance to bot canker was analyzed via frequency distribution analysis, and microsatellite and AFLP markers linked to the resistance loci were screened. From the binary frequency distribution patterns, it was found that the segregation ratio of resistant/susceptible genotypes infected by pathogen isolates Lw023 and Ls1 was 1:15; and that by Zz26 and Mx1 was 15:1. The Variation of resistance was involved in the segregation of two to four alleles of major genes, the resistance was recessive when infected by Lw023 and Ls1, but was dominant when infected with Mx1 and Zz26. A microsatellite maker, CH02a04-450, and two AFLP markers, E-AG/M-GAC-280 and E-AGG/M-CTT-110, were identified, and their map distances to the resistance loci were 5.1, 5.1 and 6.2 cM, respectively. The three markers are located in different linkage groups, while CH02a04-450 is on linkage group 2 or 7. E-AG/M-GAC-280 was successfully converted into SCAR 159. Finally, CH02a04-450 and SCAR 159 were re-examined in inoculated segregation population and presented a good reliability on predicting phenotypes of resistance.