文献类型： 外文期刊

作者： Jiang, Biao ¹ ; Wu, Zhi-Ming ¹ ; Lou, Qun-Feng ¹ ; Wang, Dong ¹ ; Zhang, Wan-Ping ³ ; Chen, Jin-Feng ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Key Lab So Vegetable Crop Genet Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Econ Crop Res, Shijiazhuang 050051, Peoples R China

3.Guizhou Univ, Coll Agr, Guiyang 550025, Peoples R China

关键词： Cucumis hystrix;Retrotransposons;Reverse transcriptase;Heterogeneity;Transcriptional activity

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2010 年 127 卷 1 期

页码：

收录情况： SCI

摘要： Ty1-copia retrotransposons are ubiquitous in the plant kingdom and have an important effect on genome structure and evolution Cucumis hystrix (2n = 2x = 24) is an exotic wild species of Cucumis with a number of valuable traits that are not available in cultivated cucumber However lack of the basic genome structure and evolutionary information about C hystrix has hindered its utilization in cucumber breeding and evolutionary research in the present study reverse transcriptase (RT) sequences of Ty1-copia retrotransposons were isolated from genome of C hystrix by PCR amplification using degenerate oligonucleotide primers The 24 clones obtained were different from each other and showed high heterogeneity Of the 24 sequences 13 are intact whereas the remaining sequences have a stop codon a frameshift or both Southern hybridization revealed that Ty1-copia retrotransposons distributed widely in C hystrix by multiple copies and the total number was estimated to be about 5460 as calculated by dot-blot hybridization The retrotransposons of this kind comprise approximately 8 5% of the C hystrix genome No transcription of Ty1-copia retrotransposons was detected by reverse transcriptase PCR (RT-PCR) in leaves of chilling or heat treated plants Moreover phylogenetic analysis showed that the 24 RT sequences were divided into five families and had homology with other species indicating that both vertical transmission and horizontal transmission are the source of Ty1-copia retrotransposons in C hystrix FISH with Ty1-copia retrotransposons in C hystrix revealed the retrotransposons of this kind were widely dispersed over all the chromosomes with clustering in terminal heterochromatin regions These results contribute to our understanding about the organization and evolution of C hystrix genome and will provide valuable information for the utilization of retrotransposons in C hystrix (C) 2010 Elsevier B V All rights reserved