文献类型： 外文期刊

作者： Wang Guang-peng ¹ ; Liu Qing-xiang ² ; Kong De-jun ² ;

作者机构： 1.Shenyang Agr Univ, Coll Hort, Shenyang 110161, Liaoning, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Hebei, Peoples R China

关键词： Chinese chestnut;nuclear degradation;programmed cell death (PCD)

期刊名称：IV INTERNATIONAL CHESTNUT SYMPOSIUM

ISSN： 0567-7572

年卷期： 2009 年 844 卷

页码：

收录情况： SCI

摘要： Shoot apex death was observed in Castanea mollissima Blume cv. 'Xin 12', using DNA degradation and changes in nuclear morphology as parameters. The buds in the shoot apex of this cultivar exhibited abscission and shoot apex interval death in the following year as a visible symptom of programmed cell death (PCD). The physiological mechanism of PCD in buds was studied by observation of the shoot apex, nuclear morphology and nuclear degradation. Extraction and staining of nuclei from buds in the shoot apex at various times showed that nuclear degradation occurred in the last ten days of July, prior to visible signs of bud abscission. From then on the nuclei degenerated quickly, and by the middle of August the PI-stained nuclei had hardly any fluorescence. It was concluded that PCD had happened in the buds of the shoot apex in July during the annual growth cycle, so that the buds of side shoots developed into new branches in the next year.