The Use of Ovule Embryo Culture for Obtaining Triploid Plants from Cross between Diploid and Tetraploid Grapes
文献类型： 外文期刊
作者： Zhao, Y. 1 ; Wu, Y. 1 ; Zhao, S. 1 ; Guo, Z. 1 ; Cheng, H. 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Changli Res Inst Fruit Trees, Changli 066600, Hebei, Peoples R China
关键词： Vitis sp.;ovule culture;ploidy;breeding
期刊名称：IX INTERNATIONAL CONFERENCE ON GRAPE GENETICS AND BREEDING
ISSN： 0567-7572
年卷期： 2009 年 827 卷
页码：
收录情况： SCI
摘要： Triploid grape breeding is one of the effective approaches to obtain new seedless cultivars but it is difficult to get triploid progenies by conventional methods. The present work was carried out to determine the efficiency of ovule embryo culture for obtaining triploid plants from cross between diploid and tetraploid grapes. The different stages of embryo development and abortion were investigated. The effects of sampling period, basal media, and beak-cut treatment in the crosses between diploid 'Muscat Hamburg' (V. vinifera) and tetraploid 'Kyoho' (V. vinifera x V. labrusca) grapes were studied. The optimum protocol consists in sampling ovules 71 days after pollination and using B5 medium supplemented with IAA 0.1 mg/L and IBA 0.1 mg/L. The percentage of germination and plantlet formation are 51.0% and 36.0%, respectively. The ploid level of plants was determined by flow cytometry.
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