文献类型： 外文期刊

作者： DAI, GH ¹ ; ANDARY, C ² ; MONDOLOTCOSSON, L ² ; BOUBALS, D ² ;

作者机构： 1.FAC PHARM MONTPELLIER,BOTAN PHYTOCHIM & MYCOL LAB,F-34060 MONTPELLIER 1,FRANCE

2.FAC PHARM MONTPELLIER,BOTAN PHYTOCHIM & MYCOL LAB,F-34060 MONTPELLIER 1,FRANCE; ECOLE NATL SUPER AGRON MONTIPELLIER,VITICULTURE LAB,F-34060 MONTPELLIER 1,FRANCE; HEBEI ACAD AGR & FORESTRY SCI,PLANT STRESS PHYSIOL LAB,SHIJIAZHUANG 050051,PEOPLES R CHINA

关键词： GRAPEVINE CALLUS;AERIAL HYPHAE;HISTOCHEMISTRY;SPECTROPHOTOMETRIC ANALYSIS;GALLOCATECHIN DERIVATIVES;FLAVONOIDS;SUBERIN

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 1995 年 101 卷 5 期

页码：

收录情况： SCI

摘要： Callus tissue of different grapevines (Vitis spp.) was inoculated with Plasmopara viticola. Short, highly-branched hyphae with necrosis, and long hyphae with heavy sporulation were observed on resistant and susceptible callus respectively. Thin-layer chromatography and spectrophotometric analysis showed that resistant callus contained greater quantities of gallocatechin derivatives than susceptible callus. Regression analysis between the field disease rating of each variety and its gallocatechin derivatives content indicated 92.2% correlation. Histochemical studies showed that, after infection with P. viticola, flavonoids appeared in the superficial cell walls of the callus, to a lesser degree on susceptible callus than on resistant callus. At a late stage of infection, the superficial cells of resistant callus were suberized, which did not occur in susceptible callus. This study showed that the preformed gallocatechin derivatives, the induced flavonoids and suberized superficial cells might play a role in the resistance of grapevine callus tissue to this fungus.