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Functional Characterization of 9-/13-LOXs in Rice and Silencing Their Expressions to Improve Grain Qualities

文献类型： 外文期刊

作者： RoyChowdhury, Moytri 1 ; Li, Xiaobai 2 ; Qi, Hangying 4 ; Li, Wenxu 5 ; Sun, Jian 2 ; Huang, Cheng 4 ; Wu, Dianxing 2 ;

作者机构： 1.Washington State Univ, Dept Hort, Pullman, WA 99164 USA

2.Zhejiang Univ, Int Atom Energy Agcy Collaborating Ctr, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

4.Shaoxing & Zhuji Agr Bur, Agr Extens Extending Stn, Shaoxing 312000, Zhejiang, Peoples R China

5.Henan Acad Agr Sci, Inst Wheat Res, Zhengzhou 450002, Henan, Peoples R China

期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2016 年

页码：

收录情况： SCI

摘要： Lipoxygenases (LOXs) are involved in oxidative rancidity and render rice unsuitable for human consumption. Here, RNA interference- (RNAi-) induced gene expression inhibition was used to analyze the functions of the bran/seed-specific LOXs in rice. r9-LOX1 and L-2 (9-LOX category) were the candidate genes expressing a bran/seed-specific LOX, while RCI-1 was (13-LOX category) a plastid-specific LOX. Real-time PCR showed that three LOXs were cultivar/tissue specific expression on a certain level. r9-LOX1 and L-2 were generally much higher in active bran/seed than in stabilized bran, mature seed, and regenerated plant. RCI-1 was barely expressed in seed. In transgenic lines, r9-LOX1, as well as L-2, expression was dramatically downregulated, compared to the nontransgenic controls. SPME/GC-MS analysis of r9-LOX1 RNAi transgenic lines showed 74.33% decrease in nonanal content (formed during oxidation of linoleic acid by lipoxygenase), but 388.24% increase in acetic acid and 184.84% hexanal (direct products of 13-LOX). These results indicate that r9-LOX1 positively regulates the amount of nonanal but negatively regulates acetic acid and hexanal. The negative regulation may be due to a mechanism of negative feedback between LOX family members. The information will help comprehensively understand the function of the bran/seed-specific LOXs, r9-LOX1, and improve the storage quality in the future.

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