浙江省农业科学院机构知识库

Over-expression of miR-146b and its regulatory role in intestinal epithelial cell viability, proliferation, and apoptosis in piglets

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文献类型：外文期刊

作者： Tao, Xin ¹ ; Liu, Shujie ¹ ; Men, Xiaoming ¹ ; Xu, Ziwei ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： MiR-146b;IPEC-J2;Proliferation;Apoptosis;TLR4

期刊名称：BIOLOGY DIRECT （影响因子：4.54；五年影响因子：3.551 ）

ISSN： 1745-6150

年卷期： 2017 年 12 卷

页码：

收录情况： SCI

摘要： Background: Weaning stress affects the small intestine of piglets. MiR-146b is differentially expressed in suckling and weaned piglets. In this study, we evaluated the effects of miR-146b on cell viability, proliferation, and apoptosis in IPEC-J2 cells. Results: Transfection with miR-146b mimics successfully increased miR-146b levels by 1000x (P < 0.001). The overexpression of miR-146b significantly promoted the apoptosis (P < 0.01) of IPEC-J2 cells, with no significant effects on cell viability or proliferation. MiR-146b suppressed the luciferase activity of the miR-TLR4-wt by 57% compared with the negative control, while mutation of the miR-146b binding site significantly blocked the suppressive effect (P < 0.05). Western blot results showed that TLR4 levels decreased in IPEC-J2 cells transfected with miR-146b mimics (P < 0.05). Conclusions: The over-expression of miR-146b promotes IPEC-J2 cell apoptosis. TLR4 is a direct target of miR-146b in IPEC-J2 cells.

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