浙江省农业科学院机构知识库

Senescence is delayed when ramie (Boehmeria nivea L.) is transformed with the isopentyl transferase (ipt) gene under control of the SAG12 promoter

收藏
分享
全文链接

文献类型：外文期刊

作者： An, Xia ¹ ; Zhang, Jingyu ¹ ; Liao, Yiwen ¹ ; Liu, Lijun ¹ ; Peng, Dingxiang ¹ ; Wang, Bo ¹ ;

作者机构： 1.Huazhong Agr Univ, Key Lab Crop Ecophysiol & Farming Syst Middle Rea, Minist Agr, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China

2.Zhejiang Acad Agr Sci, Flower Res & Dev Ctr, Hangzhou, Zhejiang, Peoples R China

关键词： ethylene;ipt;leaf senescence;ramie

期刊名称：FEBS OPEN BIO （影响因子：2.693；五年影响因子：2.519 ）

ISSN： 2211-5463

年卷期： 2017 年 7 卷 5 期

页码：

收录情况： SCI

摘要： Ramie is an economically important industrial fiber crop widely planted in China, India, and other Southeast Asian and Pacific Rim countries. It plays an important role in China's economy, where ramie farming, industry, and trade provide livelihood support to about five million people. However, poor fiber production resulting from leaf senescence and leaf abscission is a significant problem. In this study, we report the successful production of transgenic ramie plants which delayed leaf senescence and enhanced biomass. Transgenic ramie plants were obtained via transformation with the Agrobacterium tumefaciens strain harboring the binary vector pSG529 containing the isopentyl transferase (ipt) gene under control of the SAG12 promoter (PSAG12-ipt construct). Agrobacterium tumefaciens strain EHA105 was used for the midrib explant transformation. The transformation frequency was 28.29%. Southern blot confirmed the integration of 1-4 copies of the NPTII gene into the ramie genome in the tested lines. At the fiber maturation stage, the transgenic plants had higher photosynthesis rates, chlorophyll content (SPAD values), and stronger resistance to exogenous ethylene compared with wild-type plants.

相关文献

[1]Cloning and Expression of Key Enzyme Gene GalAT in Ramie Pectin Biosynthesis. Liu Jian-xin,Yu Chun-ming,Tang Shou-wei,Zhu Ai-guo,Wang Yan-zhou,Zhu Si-yuan,Ma Xiong-feng,Xiong He-ping,Liu Jian-xin. 2009

[2]Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Comparative Proteome Analysis of the Response of Ramie under Drought Stress. An, Xia,Zhang, Jingyu,Dai, Lunjin,Liao, Yiwen,Liu, Lijun,Wang, Bo,Peng, Dingxiang,An, Xia,Deng, Gang. 2016

[3]QTL mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits. Xu, Pei,Wu, Xiaohua,Wang, Baogen,Hu, Tingting,Lu, Zhongfu,Liu, Yonghua,Qin, Dehui,Wang, Sha,Li, Guojing. 2013

作者其他论文更多>>

Construction of cDNA library of Dalbergia odorifera induced by low temperature stress and screening of low temperature tolerant genes

作者：Li, Shaocui;An, Xia;Li, Fayong;Chen, Yining;Li, Xiaowen

关键词：
Research Progress on the Tolerance Mechanism and Technology of Bast Fiber Crops in Saline Soil

作者：Ji, Qingqing;Chen, Changli;Li, Shaocui;Luo, Xiahong;Liu, Tingting;Zou, Lina;An, Xia;Ji, Qingqing;Du, Guanghui;Chen, Jikang

关键词：Saline land; bast fiber; water and salt transport; cultivation regulation; crop adaptation; (sic)(sic)(sic); (sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic)
Comparative Analysis of the Major Phenotypic Characters of 23 Kenaf Cultivars

作者：Zhu, Ziyi;Luo, Xiahong;An, Xia;Chen, Changli;Liu, Tingting;Zou, Lina;Li, Shaocui;Zhu, Ziyi

关键词：Kenaf; phenotypic character; genetic diversity; principal component analysis; clustering analysis; germplasm resource; (sic)(sic); (sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic)
Sucrose synthase 3 improves fruit quality in grape

作者：Huang, Ting;Cheng, Yuanxin;Yang, Jungui;Zhou, Yunzhi;Wang, Bo;Zhou, Sihong;Jia, Haifeng;Zheng, Ting;Cheng, Guo;He, Jianjun;Hong, Pingjing

关键词：Grapes; VvSS3; ABA; VvSnRK1 beta; Transgene
Effect of different fertilization strategies on phenotypic traits, yield and fiber quality of kenaf (Hibiscus cannabinus L.)

作者：Zhu, Ziyi;An, Xia;Chen, Changli;Luo, Xiahong;Liu, Tingting;Zou, Lina;Li, Shaocui;Zhu, Ziyi

关键词：Kenaf; base fertilizer and topdressing ratio; phenotypic traits; fiber quality; (sic)(sic); (sic)(sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic); (sic)(sic)(sic)(sic)
Comprehensive Analysis of the Main Agronomic Traits of Different Varieties (Lines) of Jute

作者：An, Xingcai;Luo, Xiahong;An, Xia;Chen, Changli;Liu, Tingting;Zou, Lina;Li, Shaocui;An, Xingcai

关键词：Jute; agronomic traits; coefficient of variation; principal component analysis; cluster analysis; subordinate function analysis
Synthesis and Biological Evaluation of Disulfides Based on Garlic Extract as Type III Secretion System Inhibitors against Erwinia amylovora

作者：Yi, Aoyun;Chen, Liangye;Wei, Junjie;Li, Wanjun;Wang, Bo;Cui, Zi-Ning;Zhang, Zhao-Sheng;Shi, Yu;Wang, Xiangyun

关键词：Erwinia amylovora; type III secretionsystem (T3SS); fire blight; disulfide compounds; inhibitors

Senescence is delayed when ramie (Boehmeria nivea L.) is transformed with the isopentyl transferase (ipt) gene under control of the SAG12 promoter

作者其他论文 更多>>

Construction of cDNA library of Dalbergia odorifera induced by low temperature stress and screening of low temperature tolerant genes

Research Progress on the Tolerance Mechanism and Technology of Bast Fiber Crops in Saline Soil

Comparative Analysis of the Major Phenotypic Characters of 23 Kenaf Cultivars

Sucrose synthase 3 improves fruit quality in grape

Effect of different fertilization strategies on phenotypic traits, yield and fiber quality of kenaf (Hibiscus cannabinus L.)

Comprehensive Analysis of the Main Agronomic Traits of Different Varieties (Lines) of Jute

Synthesis and Biological Evaluation of Disulfides Based on Garlic Extract as Type III Secretion System Inhibitors against Erwinia amylovora

意 见 箱

作者其他论文更多>>

意见箱