您好,欢迎访问浙江省农业科学院 机构知识库!

Optimization of Catechin Nanoliposomes and Evaluation of Their Antioxidant Activity and Cytotoxicity

文献类型: 外文期刊

作者: Wu, Zhipan 1 ; Guan, Rongfa 1 ; Xiao, Chaogeng 2 ; Lyu, Fei 3 ; Cao, Guozhou 4 ; Liu, Mingqi 1 ; Gao, Jianguo 5 ;

作者机构: 1.China Jiliang Univ, Natl & Local United Engn Lab Qual Controlling Tec, Hangzhou 310018, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Univ Technol, Ocean Coll, Hangzhou 310014, Zhejiang, Peoples R China

4.Ningbo Entry Exit Inspect & Quarantine Technol Ct, Ningbo 315000, Zhejiang, Peoples R China

5.Shandong Exit & Entry Inspect & Quarantine Bur, Inspect & Quarantine Ctr, Qingdao 266002, Shandong, Peoples R China

关键词: Catechin;Nanoliposome;Response Surface Methodology;Scanning Electron Microscope;Antioxidant Activity;Cytotoxicity

期刊名称:SCIENCE OF ADVANCED MATERIALS ( 影响因子:1.474; 五年影响因子:0.908 )

ISSN: 1947-2935

年卷期: 2017 年 9 卷 5 期

页码:

收录情况: SCI

摘要: Catechin, which is a major component in green tea polyphenols, has been demonstrated to be a strong antioxidant. In this work, the effectiveness of catechin was enhanced by using liposomes. The response surface methodology was successfully used to optimize the biochemical and biotechnological processes. Optimal preparation conditions were as follows: phosphatidylcholine-to-cholesterol molar ratio: 3.13; catechin concentration: 3.83 mg/mL; and rotation speed: 30 rpm. Particle size and encapsulation efficiency were 221 +/- 4 nm and 76%+/- 1%, respectively. The modified surface structure of the optimized catechin nanoliposomes was observed by scanning electron microscopy, which showed that the prepared nanoliposomes were approximately 200 nm large, and had regular spherical shape. The antioxidant activity of the catechin nanoliposomes was evaluated. Results showed that the antioxidant activities of catechin nanoliposomes for the scavenging of 2,2'-azinobis-3-ethylbenzthiazoline-6-sulfonic acid, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl radicals in vitro was obviously improved. These results indicated that the liposomes protected the catechins from the influence of the external environment. Cytotoxicity over Caco-2 cells was assessed and catechin nanoliposomes were cytotoxic (a significant difference was assessed, compared with the blank control) only at high concentrations (0.025 mg/mL). In addition, higher cytotoxicity was found at 36 h than at 24 h at the same concentrations.

  • 相关文献

[1]Development and characterization of lactoferrin nanoliposome: cellular uptake and stability. Guan, Rongfa,Ma, Jieqing,Wu, Yihang,Jiang, Han,Kang, Tianshu,Lu, Fei,Xiao, Chaogeng. 2012

[2]Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles. Guan, Rongfa,Kang, Tianshu,Liu, Mingqi,Lu, Fei,Zhang, Zhiguo,Shen, Haitao. 2012

[3]Combined cytotoxic effects of pesticide mixtures present in the Chinese diet on human hepatocarcinoma cell line. Ma, Mengmeng,Chen, Chen,Yang, Guiling,Li, Yun,Chen, Zhijun,Qian, Yongzhong,Yang, Guiling.

[4]Inhibition of growth and induction of apoptosis in A549 cells by compounds from oxheart cabbage extract. Wang, Wei,Wang, Nan,Liu, Caiqin,Jin, Jianchang,Shao, Bo,Shen, Lianqing.

[5]Extrusion Process Optimization for Soluble Dietary Fibers Production From Agrocybe Cylindracea Using Response Surface Methodology. Lv, Guoying,Zhang, Zuofa,Pan, Huijuan,Fan, Leifa. 2012

[6]Response surface methodology to design a selective co-enrichment broth of Escherichia coli, Salmonella spp. and Staphylococcus aureus for simultaneous detection by multiplex PCR. Zhang, Qiao Yan,Zhou, Yu,Wang, Xiao Fu,Xu, Jun Feng,Zhou, Wen Wu. 2012

[7]Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19. Li, Yin,Cui, Fengjie,Liu, Zhiqiang,Ping, Lifeng,Xue, Yaping,Liu, Zhiqiang,Zhao, Hui,Ping, Lifeng,Yang, Yinan,Yan, Lijiao. 2009

[8]Response surface methodology to design a selective enrichment broth for rapid detection of Salmonella spp. by SYBR Green I (TM) real-time PCR. Zhang, Qiaoyan,Wang, Xiaofu,Chen, Tingting,Yang, Shengli,Guo, Hui. 2013

[9]Optimization of Conditions for Cyanidin-3-O-Glucoside (C3G) Nanoliposome Production by Response Surface Methodology and Cellular Uptake Studies in Caco-2 Cells. Liang, Tisong,Guan, Rongfa,Liu, Mingqi,Shen, Haitao,Xia, Qile.

[10]Comparative study on antioxidant activity of four varieties of Flammulina velutipes with different colour. Zhang, Zuofa,Jin, Qunli,Lv, Guoying,Fan, Lijun,Pan, Huijuan,Fan, Leifa. 2013

[11]Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi). Zhang, Zuofa,Jin, Jie,Shi, Liangen,Zhang, Zuofa. 2009

[12]Improvement of the Nutritional Quality of Cottonseed Meal by Bacillus subtilis and the Addition of Papain. Sun, Hong,Feng, Jie,Tang, Jiang-Wu,Yao, Xiao-Hong,Wu, Yi-Fei,Wang, Xin. 2012

[13]Optimization of the Microwave-Assisted Extraction Process for Polysaccharides in Himematsutake (Agaricus blazei Murrill) and Evaluation of Their Antioxidant Activities. Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Fan, Leifa,Shi, Liangen. 2011

[14]Antioxidant and anti-fatigue effects of anthocyanins of mulberry juice purification (MJP) and mulberry marc purification (MMP) from different varieties mulberry fruit in China. Jiang, Dong-Qing,Xu, Dian-Hong,Huang, Ya-Si,Yuan, Ke,Guo, Ying,Lv, Zhi-Qiang. 2013

[15]Antioxidant activities of polysaccharides of Flammulina velutipes obtained by ultra filtration. He, Weiqiang,Zhang, Zuofa. 2013

[16]Analysis and comparison of the active components and antioxidant activities of extracts from Abelmoschus esculentus L. Liao, Haibing,Liu, Hualiang,Yuan, Ke,Liao, Haibing,Shi, Xiangjun,Dong, Wenqi. 2012

[17]Antioxidant and hepatoprotective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey. Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Fan, Leifa,Pandey, Ashok,He, Weiqiang.

[18]Effects of Different Drying Methods and Extraction Condition on Antioxidant Properties of Shiitake (Lentinus edodes). Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Wu, Yongzhi,Fan, Leifa.

[19]Sonication inhibited browning but decreased polyphenols contents and antioxidant activity of fresh apple (malus pumila mill, cv. Red Fuji) juice. Sun, Yujing,Zhong, Liezhou,Lin, Wenwen,Ye, Xingqian,Cao, Lianfei.

[20]Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes. Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Fan, Leifa,He, Weiqiang,Shi, Liangen.

作者其他论文 更多>>