文献类型： 外文期刊

作者： Nekrasov, Vladimir ¹ ; Wang, Congmao ² ; Win, Joe ¹ ; Lanz, Christa ³ ; Weigel, Detlef ³ ; Kamoun, Sophien ¹ ;

作者机构： 1.Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

2.Zhejiang Acad Agr Sci, Inst Digital Agr, Hangzhou 310021, Zhejiang, Peoples R China

3.Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany

4.Rothamsted Res, Harpenden AL5 2JQ, Herts, England

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non- transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/ Cas9 technology. We used wholegenome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/ Cas9 being a highly precise tool, as we did not detect off- target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.