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RNA-Sequencing Reveals Biological Networks during Table Grapevine ('Fujiminori') Fruit Development

文献类型： 外文期刊

作者： Shangguan, Lingfei 1 ; Mu, Qian 1 ; Fang, Xiang 1 ; Zhang, Kekun 1 ; Jia, Haifeng 1 ; Li, Xiaoying 3 ; Bao, Yiqun 4 ; Fan 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing, Jiangsu, Peoples R China

2.Shandong Acad Grape, Jinan, Shandong, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China

4.Nanjing Agr Univ, Coll Life Sci, Nanjing, Jiangsu, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Grapevine berry development is a complex and genetically controlled process, with many morphological, biochemical and physiological changes occurring during the maturation process. Research carried out on grapevine berry development has been mainly concerned with wine grape, while barely focusing on table grape. 'Fujiminori' is an important table grapevine cultivar, which is cultivated in most provinces of China. In order to uncover the dynamic networks involved in anthocyanin biosynthesis, cell wall development, lipid metabolism and starch-sugar metabolism in 'Fujiminori' fruit, we employed RNA-sequencing (RNA-seq) and analyzed the whole transcriptome of grape berry during development at the expanding period (40 days after full bloom, 40DAF), veraison period (65DAF), and mature period (90DAF). The sequencing depth in each sample was greater than 12x, and the expression level of nearly half of the expressed genes were greater than 1. Moreover, greater than 64% of the clean reads were aligned to the Vitis vinifera reference genome, and 5,620, 3,381, and 5,196 differentially expressed genes (DEGs) were identified between different fruit stages, respectively. Results of the analysis of DEGs showed that the most significant changes in various processes occurred from the expanding stage to the veraison stage. The expression patterns of F3'H and F3'5'H were crucial in determining red or blue color of the fruit skin. The dynamic networks of cell wall development, lipid metabolism and starch-sugar metabolism were also constructed. A total of 4,934 SSR loci were also identified from 4,337 grapevine genes, which may be helpful for the development of phylogenetic analysis in grapevine and other fruit trees. Our work provides the foundation for developmental research of grapevine fruit as well as other non-climacteric fruits.

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