Marker development using SLAF-seq and whole-genome shotgun strategy to fine-map the semi-dwarf gene ari-e in barley
文献类型： 外文期刊
作者： Jia, Qiaojun 1 ; Tan, Cong 3 ; Wang, Junmei 4 ; Zhang, Xiao-Qi 3 ; Zhu, Jinghuan 4 ; Luo, Hao 3 ; Yang, Jianming 4 ; West 1 ;
作者机构： 1.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
2.Key Lab Plant Secondary Metab & Regulat Zhejiang, Hangzhou 310018, Zhejiang, Peoples R China
3.Murdoch Univ, Western Barley Genet Alliance, Murdoch, WA 6150, Australia
4.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China
5.Govt Western Australia, Dept Agr & Food, S Perth, WA 6155, Australia
6.InterGrain Pty Ltd, 19 Ambitious Link, Bibra Lake, WA 6163, Australia
关键词： Barley;Semi-dwarf;SLAF;Whole-genome sequence;Fine-map
期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）
ISSN： 1471-2164
年卷期： 2016 年 17 卷
页码：
收录情况： SCI
摘要： Background: Barley semi-dwarf genes have been extensively explored and widely used in barley breeding programs. The semi-dwarf gene ari-e from Golden Promise is an important gene associated with some agronomic traits and salt tolerance. While ari-e has been mapped on barley chromosome 5H using traditional markers and next-generation sequencing technologies, it has not yet been finely located on this chromosome. Results: We integrated two methods to develop molecular markers for fine-mapping the semi-dwarf gene ari-e: (1) specific-length amplified fragment sequencing (SLAF-seq) with bulked segregant analysis (BSA) to develop SNP markers, and (2) the whole-genome shotgun sequence to develop InDels. Both SNP and InDel markers were developed in the target region and used for fine-mapping the ari-e gene. Linkage analysis showed that ari-e co-segregated with marker InDel-17 and was delimited by two markers (InDel-16 and DGSNP21) spanning 6.8 cM in the doubled haploid (DH) Dash x VB9104 population. The genetic position of ari-e was further confirmed in the Hindmarsh x W1 DH population which was located between InDel-7 and InDel-17. As a result, the overlapping region of the two mapping populations flanked by InDel-16 and InDel-17 was defined as the candidate region spanning 0.58 Mb on the POPSEQ physical map. Conclusions: The current study demonstrated the SLAF-seq for SNP discovery and whole-genome shotgun sequencing for InDel development as an efficient approach to map complex genomic region for isolation of functional gene. The ari-e gene was fine mapped from 10 Mb to 0.58 Mb interval.
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