您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Stable Transfection of Eimeria intestinalis and Investigation of Its Life Cycle, Reproduction and Immunogenicity

文献类型： 外文期刊

作者： Shi, Tuanyuan 1 ; Tao, Geru 2 ; Bao, Guolian 1 ; Suo, Jingxia 2 ; Hao, Lili 3 ; Fu, Yuan 1 ; Suo, Xun 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Dept Anim Parasitol, Hangzhou, Zhejiang, Peoples R China

2.China Agr Univ, Coll Vet Med, Natl Anim Protozoa Lab, Beijing 100094, Peoples R China

3.Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu, Peoples R China

关键词： Eimeria intestinalis;YFP;stable transfection;life cycle;reproduction;pathogenicity

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Rabbit coccidiosis, caused by infection of Eimeria spp. is one of the most severe parasitic diseases in rabbits. Eimeria intestinalis is one of the most immunogenic species in rabbit coccidia. Due to the lack of genomic information and unsuccessful in vitro cultivation, genetic manipulation of rabbit coccidia lagged behind other apicomplexan parasites. Using regulatory sequences from E tenella, we obtained a transgenic line of E. intestinalis expressing yellow fluorescent protein (YFP). YFP was continuously expressed throughout the whole life cycle. Morphological features of E. intestinalis in different developmental stages were dynamically observed with the transgenic line. Some important features in the endogenous development stages were observed. Trophozoites were found as early as 4 h post inoculation. Two types of schizonts and merozoites were observed in first three of the four schizogonies. Beside jejunum and ileum, gametogony stage and oocysts were also found in the duodenum and vermiform appendix. In addition, the transgenic strain was highly immunogenic but less pathogenic than the wild type. Considering the high immunogenicity of E. intestinalis and amenability to transfection with foreign genes, transgenic E. intestinalis could be a promising oral eukaryotic vaccine vector.

  • 相关文献

[1]Transgenic Eimeria magna Perard, 1925 Displays similar Parasitological Properties to the Wild-type strain and induces an exogenous Protein-specific immune response in rabbits (Oryctolagus cuniculus L.). Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Shi, Tuanyuan,Duszynski, Donald W.. 2017

[2]Southern rice black-streaked dwarf virus (SRBSDV) directly affects the feeding and reproduction behavior of its vector, Sogatella furcifera (Horvath) (Hemiptera: Delphacidae). Xu, Hongxing,Zheng, Xusong,Yang, Yajun,Tian, Junce,Lu, Zhongxian,He, Xiaochan. 2014

[3]Irradiation as a quarantine treatment for the solenopsis mealybug, Phenacoccus solenopsis. Huang, Fang,Li, Weidi,Bei, Yawei,Lin, Wencai,Lu, Yaobin,Li, Xiuqiong,Wang, Bingkui.

[4]Dietary daidzein influences laying performance of ducks (Anas platyrhynchos) and early post-hatch growth of their hatchlings by modulating gene expression. Zhao, RQ,Wang, YJ,Zhou, YC,Ni, YD,Lu, LZ,Grossmann, R,Chen, J.

[5]Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp avenae Strain RS-2. Masum, Md. Mahidul Islam,Yang, Yingzi,Li, Bin,Olaitan, Ogunyemi Solabomi,Chen, Jie,Zhang, Yang,Fang, Yushi,Qiu, Wen,Masum, Md. Mahidul Islam,Wang, Yanli,Sun, Guochang. 2017

[6]The regulatory factor X protein MoRfx1 is required for development and pathogenicity in the rice blast fungus Magnaporthe oryzae. Sun, Dandan,Cao, Huijuan,Shi, Yongkai,Huang, Pengyun,Lu, Jianping,Cao, Huijuan,Liu, Xiaohong,Lin, Fucheng,Dong, Bo. 2017

[7]Autophagy vitalizes the pathogenicity of pathogenic fungi. Liu, Xiao-Hong,Gao, Hui-Min,Lin, Fu-Cheng,Lu, Jian-Ping,Xu, Fei,Devenish, Rodney J.,Devenish, Rodney J.,Lin, Fu-Cheng. 2012

[8]Characterization of 47 Cys(2)-His(2) zinc finger proteins required for the development and pathogenicity of the rice blast fungus Magnaporthe oryzae. Cao, Huijuan,Huang, Pengyun,Zhang, Lilin,Shi, Yongkai,Sun, Dandan,Yan, Yuxin,Lu, Jianping,Cao, Huijuan,Liu, Xiaohong,Lin, Fucheng,Dong, Bo,Chen, Guoqing,Snyder, John Hugh.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台