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Recombinant Lactococcus lactis expressing porcine insulin-like growth factor I ameliorates DSS-induced colitis in mice

文献类型： 外文期刊

作者： Liu, Shujie 1 ; Li, Yongming 1 ; Deng, Bo 1 ; Xu, Ziwei 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, 145 Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Insulin-like growth factor I;Lactococcus lactis;Colitis;Intestinal repair

期刊名称：BMC BIOTECHNOLOGY （ 影响因子：2.563； 五年影响因子：3.292 ）

ISSN： 1472-6750

年卷期： 2016 年 16 卷

页码：

收录情况： SCI

摘要： Background: Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits its research opportunities and practical application in the fields of medicine and animal husbandry. In this study, a tandem repeat strategy was used to express three copies of the same pIGF-I-3 protein in L. lactis. The activity of recombinant pIGF-I-3 (rpIGF-I-3) was further examined by a mouse model of dextran sulfate sodium (DSS)-induced colitis. In addition, the potential of recombinant L. lactis expressing pIGF-I-3 to reduce inflammatory disease was evaluated. Results: pIGF-I-3 could be expressed in L. lactis by the detection of SDS-PAGE and Western blot. Experimental colitis was induced in BALB/c mice by administration of 5 % DSS in drinking water, and the clinical symptoms were observed in DSS-treated mice. Oral administration of recombinant L. lactis expressing pIGF-I-3 improved the colonic architecture, and significantly reduced the increase of colonic damage score (P < 0.05). Furthermore, recombinant L. lactis expressing pIGF-I-3 treatment significantly reduced serum DAO activity and colonic MPO level, and elevated colonic occludin level compared to the DSS group (P < 0.05). Conclusions: The pIGF-I-3 expressed in L. lactis has good biological activity, and oral administration of recombinant L. lactis expressing pIGF-I-3 attenuated the symptoms and development of DSS-induced colitis in mice. These suggested that L. lactis could be a potential host bacterium for production and delivery of IGF-I against intestinal diseases.

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[1]产肠毒素大肠杆菌K88黏附素蛋白亚基Fae G在L.lactis中的表达及其抗原性. 刘淑杰,李永明,徐子伟,王一成. 2009

[2]Purified PEGylated porcine glucagon-like peptide-2 reduces the severity of colonic injury in a murine model of experimental colitis. Qi, Ke-ke,Wu, Jie,Wan, Jing,Men, Xiao-ming,Xu, Zi-wei.

[3]Induction of specific immune responses in piglets by intramuscular immunization with fimbrial adhesin FaeG expressed in Lactococcus lactis. Liu, Shujie,Li, Yongming,Xu, Ziwei. 2013

[4]Subcutaneous or Oral Immunization of Mice with Lactococcus lactis Expressing F4 Fimbrial Adhesin FaeG. Liu, Shujie,Li, Yongming,Xu, Ziwei,Wang, Yicheng. 2013

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