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Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins

文献类型： 外文期刊

作者： Liu, Yan 1 ; Chen, Hui 1 ; Wei, Qiang 1 ; Xiao, Chenwen 1 ; Ji, Quanan 1 ; Bao, Guolian 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Anim Husb & Vet Inst, Hangzhou, Zhejiang, Peoples R China

关键词： Bordetella bronchiseptica;Recombinant proteins;Immune-protective protein;Subunit vaccine

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2015 年 11 卷

页码：

收录情况： SCI

摘要： Background: The Gram-negative pathogen Bordetella bronchiseptica causes acute and chronic respiratory infection in a variety of animals. Currently, there is no vaccine to prevent these infections. To identify useful candidate antigens for such a vaccine, five B. bronchiseptica genes including amino acid ATP-binding cassette transporter substrate-binding protein (ABC), lipoprotein (PL), outer membrane porin protein (PPP), leu/ile/val-binding protein (BPP), and conserved hypothetical protein (CHP) were cloned and the recombinant proteins were expressed. The immune responses of mice to vaccination with individual recombinant proteins were measured. Results: Each of the tested recombinant proteins induced a high antibody titer. PPP and PL showed protective indices against challenges with B. bronchiseptica. The protection ratios were 62.5 and 50 %, respectively, compared with 12.5 % for control vaccinations. The protection ratios of ABC, BPP, and CHP were not significantly different from the controls. IgG-subtype and cytokine analysis demonstrated that PPP and PL can induce two immune responses: a humoral immune response and a cell-mediated immune response. The humoral immunity-mediated, Th2-type response dominated. Conclusion: The identification of PPP and PL, which offer immune-protective potential, identifies them as candidates for the development of a diagnostic test or a vaccine for B. bronchiseptica.

  • 相关文献

[1]Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins. Liu Yan,Qin Feng-yan,Bao Guo-lian,Chen Hui,Xiao Chen-wen,Wei Qiang,Ji Quan-an. 2014

[2]Greater efficacy of the ECMS-oil adjuvant over other formulations on immune responses against Bordetella bronchiseptica in rabbits and the underlying mechanism. Xiao, Chenwen,Bao, Guolian,Liu, Yan,Wei, Qiang,Ji, Quanan,Liu, Yaping,Pan, Lijun.

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