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MOLECULAR CHARACTERIZATION OF THE sigma C-ENCODING GENE FROM NOVEL DUCK REOVIRUS

文献类型： 外文期刊

作者： Yun, T. 1 ; Chen, H. 2 ; Hua, J. 1 ; Yu, B. 1 ; Chen, L. 1 ; Ni, Z. 1 ; Ye, W. 1 ; Zhang, C. 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

2.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China

关键词： Novel duck reovirus;sigma C-encoding gene;Expression;Phylogenetic analysis;Genotype

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （ 影响因子：0.49； 五年影响因子：0.686 ）

ISSN： 1018-7081

年卷期： 2015 年 25 卷 3 期

页码：

收录情况： SCI

摘要： We cloned and sequenced the sigma C-encoding gene of a novel duck reovirus (N-DRV) isolate ZJ00M. The sigma C gene is 966 bp long and is encoded by the third ORF of the S1 segment, a small protein of 321 amino acid residues (approximately 34.1 kDa). Expressed sigma C fusion protein in E. coli showed good antigenicity and immunogenicity in a western blotting assay and indirect immunofluorescence assays (IFA). The comparative sequence analyses indicated that N-DRV sigma C shared high identity levels with ARV species (classical MDRV and ARV) and a lower degree of identity with other orthoreovirus counterparts, but the sequence identities were no more than 45 %. As observed in classical MDRV and ARV sigma C protein sequences, some sequence motifs, conserved sequence motifs and/or functional domains were identified in the sequence of N-DRV sigma C. The phylogenetic analysis of sigma C/sigma 1 sequence revealed that N-DRV was different from other orthoreovirus species groups but remained classified into the avian orthoreoviruses along with other ARVs, suggesting that N-DRV and classical MDRVs constitute a separate geno group relative to ARV. These results are important for future studies examining the structure, function and immunogenicity of N-DRV sigma C.

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