文献类型： 外文期刊

作者： Zhu Fengxiang ¹ ; Yao Yanlai ¹ ; Wei Huanzhang ² ; Hong Chunlai ¹ ; Ping Lifeng ¹ ; Xue Zhiyong ¹ ; Chen Xiaoyang ¹ ; Yan ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

2.Agr Tech Extens Ctr, Ninghai 315600, Zhejiang, Peoples R China

关键词： cellulose-decomposer microorganism;Penicillium oxalicum;simulated composting

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （ 影响因子：0.49； 五年影响因子：0.686 ）

ISSN： 1018-7081

年卷期： 2015 年 25 卷 3 期

页码：

收录情况： SCI

摘要： The aim of this study was to screen cellulose-decomposer microorganisms and to study the effects of dehydration in simulated composting. Cellulose-decomposer microorganisms were first isolated from fresh cow dung and cow dung natural compost in different stages by the cellulose-Congo Red medium. The obtained strains were further screened for a high ability to decompose cellulose by detecting the cellulose-enzyme activity and by observing the disintegration of filter paper and straw. A strong cellulose-decomposer microorganism was finally obtained and was identified as Penicillium oxalicum F12. The following aspects were experimentally determined to be the optimal conditions: a wheat bran and microcrystalline cellulose mix (4: 1) as the carbon source, ammonium sulfate as the nitrogen source, pH of 5 similar to 6, 5% inoculum size, incubation at 30 similar to 35 degrees C for 120h. Under these conditions, the CMCase of strain F12 was 47.50 IU.mL-1, and the FPA was 11.10 IU.mL-1. In a dehydration test of simulated composting, the water content of the inoculated processing residue material was decreased by 3.9% as compared with that of the control residue materials. The dehydration volume was 47.6g, which accounted for 19.8% of the initial material moisture content. Penicillium oxalicum F12 was a strong cellulose-decomposer microorganism with broad development potential.