Dynamic Metabolic and Transcriptional Profiling of Rhodococcus sp Strain YYL during the Degradation of Tetrahydrofuran
文献类型： 外文期刊
作者： He, Zhixing 1 ; Yao, Yanlai 2 ; Lu, Zhenmei 1 ; Ye, Yangfang 3 ;
作者机构： 1.Zhejiang Univ, Coll Life Sci, Hangzhou 310003, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Environm Resource Soil & Fertilizer, Hangzhou, Zhejiang, Peoples R China
3.Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）
ISSN： 0099-2240
年卷期： 2014 年 80 卷 9 期
页码：
收录情况： SCI
摘要： Although tetrahydrofuran-degrading Rhodococcus sp. strain YYL possesses tetrahydrofuran (THF) degradation genes similar to those of other tetrahydrofuran-degrading bacteria, a much higher degradation efficiency has been observed in strain YYL. In this study, nuclear magnetic resonance (NMR)-based metabolomics analyses were performed to explore the metabolic profiling response of strain YYL to exposure to THF. Exposure to THF slightly influenced the metabolome of strain YYL when yeast extract was present in the medium. The metabolic profile of strain YYL over time was also investigated using THF as the sole carbon source to identify the metabolites associated with high-efficiency THF degradation. Lactate, alanine, glutarate, glutamate, glutamine, succinate, lysine, trehalose, trimethylamine-N-oxide (TMAO), NAD(+), and CTP were significantly altered over time in strain YYL grown in 20 mM THF. Real-time quantitative PCR (RT-qPCR) revealed changes in the transcriptional expression levels of 15 genes involved in THF degradation, suggesting that strain YYL could accumulate several disturbances in osmoregulation (trehalose, glutamate, glutamine, etc.), with reduced glycolysis levels, an accelerated tricarboxylic acid cycle, and enhanced protein synthesis. The findings obtained through H-1 NMR metabolomics analyses and the transcriptional expression of the corresponding genes are complementary for exploring the dynamic metabolic profile in organisms.
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