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A low-virulence Eimeria intestinalis isolate from rabbit (Oryctolagus cuniculus) in China: molecular identification, pathogenicity, and immunogenicity

文献类型： 外文期刊

作者： Shi, Tuanyuan 1 ; Bao, Guolian 1 ; Fu, Yuan 1 ; Suo, Xun 2 ; Hao, Lili 3 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Dept Anim Parasitol, Hangzhou 310021, Zhejiang, Peoples R China

2.China Agr Univ, Coll Vet Med, Parasitol Lab, Beijing 100094, Peoples R China

3.Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Peoples R China

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2014 年 113 卷 3 期

页码：

收录情况： SCI

摘要： An Eimeria intestinalis isolated from a rabbit in China was first identified by amplifying the 18S small subunit (SSU) ribosomal RNA gene. The size of the amplified fragment was 1521 bp. The 18S SSU RNA gene of the E. intestinalis isolate shared 99 % sequence identity with E. intestinalis isolates from France and the Czech Republic, with 100 and 96 % coverage, respectively. Then, the pathogenicity and immunogenicity of the E. intestinalis isolate were evaluated in specific pathogen free (SPF) rabbits. In the pathogenicity assay, SPF rabbits in four groups were infected with 5x10(3), 5x10(4), 5x10(5), and 0 sporulated oocysts, respectively. Clinical signs including diarrhoea, constipation, loss of appetite, and reduction of body weight gain were observed in rabbits inoculated with 5x10(4) and 5x10(5) oocysts. And one rabbit (25 %) inoculated with 5x10(5) oocysts died 15 days after the inoculation. In the immunogenicity assay, SPF rabbits in five groups (named B1, B2, B3, B4, and B5) were immunised with 5x10(1), 5x10(2), 5x10(3), 0, and 0 sporulated oocysts, respectively. All rabbits but the B5 group were challenged with 1x10(6) oocysts. After the challenge, no or slight clinical signs were seen in rabbits of the B2 and B3 groups. Compared with the control, a 69.6 and 84.5 % reduction of oocyst output was observed in the B2 and B3 groups, respectively. The body weight gain of the two groups was obviously higher than that of the challenge control group. All the results show that the E. intestinalis isolate has low virulence but immunogenicity in rabbit.

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