C-13 pulse-chase labeling comparative assessment of the active methanogenic archaeal community composition in the transgenic and nontransgenic parental rice rhizospheres
文献类型： 外文期刊
作者： Zhu, Weijing 1 ; Lu, Haohao 2 ; Hill, Jaclyn 3 ; Guo, Xiaohui 1 ; Wang, Hailong 4 ; Wu, Weixiang 1 ;
作者机构： 1.Zhejiang Univ, Coll Environm & Resource Sci, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Environm Resource Soil & Fertilizer, Hangzhou, Zhejiang, Peoples R China
3.Rhodes Univ, Dept Zool & Entomol, ZA-6140 Grahamstown, South Africa
4.Zhejiang A&F Univ, Sch Environm & Resource Sci, Hangzhou, Zhejiang, Peoples R China
关键词： Bt transgenic rice;C-13 allocation;clone library;methanogenic archaeal community structure
期刊名称：FEMS MICROBIOLOGY ECOLOGY （ 影响因子：4.194； 五年影响因子：5.137 ）
ISSN： 0168-6496
年卷期： 2014 年 87 卷 3 期
页码：
收录情况： SCI
摘要： More and more investigations indicate that genetic modification has no significant or persistent effects on microbial community composition in the rice rhizosphere. Very few studies, however, have focused on its impact on functional microorganisms. This study completed a C-13-CO2 pulse-chase labeling experiment comparing the potential effects of cry1Ab gene transformation on C-13 tissue distribution and rhizosphere methanogenic archaeal community composition with its parental rice variety (Ck) and a distant parental rice variety (Dp). Results showed that C-13 partitioning in aboveground biomass (mainly in stems) and roots of Dp was significantly lower than that of Ck. However, there were no significant differences in C-13 partitioning between the Bt transgenic rice line (Bt) and Ck. RNA-stable isotope probing combined with clone library analyses inferred that the group Methanosaetaceae was the predominant methanogenic Archaea in all three rice rhizospheres. The active methanogenic archaeal community in the Bt rhizosphere was dominated by Methanosarcinaceae, Methanosaetaceae, and Methanomicrobiaceae, while there were only two main methanogenic clusters (Methanosaetaceae and Methanomicrobiaceae) in the Ck and Dp rhizospheres. These results indicate that the insertion of cry1Ab gene into the rice genome has the potential to result in the modification of methanogenic community composition in its rhizosphere.
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