Expression of mucoid induction factor MucE is dependent upon the alternate sigma factor AlgU in Pseudomonas aeruginosa
文献类型： 外文期刊
作者： Yin, Yeshi 1 ; Damron, F. Heath 1 ; Withers, T. Ryan 1 ; Pritchett, Christopher L. 5 ; Wang, Xin 3 ; Schurr, Michae 1 ;
作者机构： 1.Marshall Univ, Joan C Edwards Sch Med, Dept Biochem & Microbiol, Huntington, WV 25755 USA
2.Marshall Univ, Joan C Edwards Sch Med, Dept Pediat, Huntington, WV 25755 USA
3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China
4.Progenesis Technol LLC, Huntington, WV 25701 USA
5.Univ Colorado, Sch Med, Dept Microbiol, Aurora, CO 80045 USA
6.E Tennessee State Univ, Dept Hlth Sci, Johnson City, TN 37615 USA
7.Univ Virginia, Div Infect Dis & Int Hlth, Charlottesville, VA 22908 USA
关键词： Pseudomonas aeruginosa;Alginate;Mucoidy;AlgU/T;MucE;Sigma factor
期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）
ISSN： 1471-2180
年卷期： 2013 年 13 卷
页码：
收录情况： SCI
摘要： Background: Alginate overproduction in P. aeruginosa, also referred to as mucoidy, is a poor prognostic marker for patients with cystic fibrosis (CF). We previously reported the construction of a unique mucoid strain which overexpresses a small envelope protein MucE leading to activation of the protease AlgW. AlgW then degrades the anti-sigma factor MucA thus releasing the alternative sigma factor AlgU/T (sigma(22)) to initiate transcription of the alginate biosynthetic operon. Results: In the current study, we mapped the mucE transcriptional start site, and determined that P-mucE activity was dependent on AlgU. Additionally, the presence of triclosan and sodium dodecyl sulfate was shown to cause an increase in P-mucE activity. It was observed that mucE-mediated mucoidy in CF isolates was dependent on both the size of MucA and the genotype of algU. We also performed shotgun proteomic analysis with cell lysates from the strains PAO1, VE2 (PAO1 with constitutive expression of mucE) and VE2 Delta algU (VE2 with in-frame deletion of algU). As a result, we identified nine algU-dependent and two algU-independent proteins that were affected by overexpression of MucE. Conclusions: Our data indicates there is a positive feedback regulation between MucE and AlgU. Furthermore, it seems likely that MucE may be part of the signal transduction system that senses certain types of cell wall stress to P. aeruginosa.
- 相关文献
作者其他论文 更多>>
-
Insect residual streams supplement improves chili pepper growth: Insights into the role of rhizosphere soil microbiome and metabolome
作者：Zhou, Hanghai;Zheng, Xusong;Shen, Qi;Yao, Xiaohong;Sun, Hong;Wang, Xin;Wu, Yifei;Tang, Jiangwu;Zhu, Zhigang;Yang, Chenghu;Li, Hua;Liu, Yuxue;Jiang, Lijia;Zhang, Chunfang
关键词：Insect farming; Insect residual stream; Chili pepper; Plant growth; Rhizosphere microbial community; Metabolic profile
-
Integrated Analysis of the Transcriptome and Metabolome Reveals the Network Regulating Fruit Taste in Sponge Gourd (Luffa cylindrica)
作者：Chai, Yaqian;Qiu, Wenjing;Gao, Luyao;Dong, Wenqi;Zhang, Peng;Zhou, Shengjun;Wang, Xin;Zhu, Yuqiang;Sun, Yuyan;Chai, Yaqian;Li, Zhikun
关键词：sponge gourd; taste; transcriptome; metabolome; mechanism
-
Enhancing Akkermansia growth via phytohormones: a strategy to modulate the gut-bone axis in postmenopausal osteoporosis therapy
作者：Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Sun, Haibiao;Han, Xiaoqiang;Deng, Yixuan;Li, Li
关键词：Osteoporosis; Phytohormones; Soybean isoflavone; Naringin; Denosumab; Gut microbiome
-
Raffinose-metabolizing bacteria impair radiation-associated hematopoietic recovery via the bile acid/FXR/NF-κB signaling pathway
作者：Jiao, Yang;Ren, Jiawei;Yuan, Nan;Shen, Jiaqi;Guo, Hongjuan;Cao, Jianping;Wu, Depei;Jiao, Yang;Ren, Jiawei;Yuan, Nan;Guo, Hongjuan;Cao, Jianping;Jiao, Yang;Wu, Depei;Qi, Xiaofei;Xie, Shichang;Zhou, Zhemin;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Yin, Huafang;Wang, Jian;Wang, Xin;Wang, Xin;Zhou, Zhemin;Zhou, Zhemin
关键词：Radiation-induced hematopoietic injury; gut microbiome;
Bacteroides acidifaciens ; raffinose-metabolizing bacteria; Intestinal-Hematopoietic Axis; UDCA; FXR -
Soil heterogeneity influences the biotoxicity of photoaged tire wear particles in Eisenia fetida: A comparative assessment
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Yao, Xiaohong;Wang, Yuan;Jin, Junwei;Tang, Jiangwu;Wu, Zhou;Li, Yongfu;Zhou, Jianjiang;Jiang, Lijia;Zhang, Chunfang
关键词：Tire wear particle; Photoaging; Earthworm; Soil heterogeneity; Oxidative damage
-
Regulatory Roles of Quercetin in Alleviating Fructose-Induced Hepatic Steatosis: Targeting Gut Microbiota and Inflammatory Metabolites
作者：Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Li, Xinxin;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Deng, Yixuan;Li, Li
关键词：fructose; gut microbiota; hepatic steatosis; quercetin
-
6PPD-quinone exposure induces oxidative damage and physiological disruption in Eisenia fetida: An integrated analysis of phenotypes, multi-omics, and intestinal microbiota
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Wang, Yuan;Yao, Xiaohong;Tang, Jiangwu;Wu, Zhou;Wu, Zhou;Jiang, Lijia;Zhang, Chunfang;Yan, Zhongyong
关键词：Earthworm; 6PPD-quinone; Oxidative stress; Multi-omics; Intestinal microbial community
