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Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro

文献类型： 外文期刊

作者： Ma, Jieqing 1 ; Guan, Rongfa 1 ; Shen, Haitao 2 ; Lu, Fei 3 ; Xiao, Chaogeng 4 ; Liu, Mingqi 1 ; Kang, Tianshu 1 ;

作者机构： 1.China Jiliang Univ, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Hangzhou 310018, Peoples R China

2.Zhejiang Prov Ctr Dis Prevent & Control, Hangzhou 310051, Zhejiang, Peoples R China

3.Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Lactoferrin nanoliposomes;Caco-2;Cell viability;MTT

期刊名称：FOOD AND CHEMICAL TOXICOLOGY （ 影响因子：6.023； 五年影响因子：5.844 ）

ISSN： 0278-6915

年卷期： 2013 年 59 卷

页码：

收录情况： SCI

摘要： The anticancer activities of Lactoferrin (Lf) and Lf nanoliposomes in Caco-2 cells were observed in this study, and mitochondrial function (MTT assay), count kit-8(CCK-8), detection of intracellular reactive oxygen species (ROS) and apoptosis induction (AO/EB staining) assays were used to evaluate the anticancer activity. MTT results demonstrated that Lf nanoliposomes and Lf reduced the mitochondrial activity of cells in a manner of dose and time effect, and the viabilities of Caco-2 cell were significantly decreased in vitro following exposure to Lf nanoliposomes at the concentrations of 5 and 10 mg/mL. LDH leakage and ROS significantly increased in cells exposed to Lf nanoliposomes ( >= 5 mg/mL), while Lf induced ROS only at higher doses (10 mg/mL). CCK-8 evaluation of cell proliferation and AO/EB double staining supported the anti-proliferative effects of Lf liposomes. Our findings demonstrated that the presence of Lf nanoliposome is more significant than Lf in inhibiting human tumor cells proliferation. Therefore, it can be concluded that Lf nanoliposomes are a potential therapeutic modality in the management of tumors. (c) 2013 Elsevier Ltd. All rights reserved.

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