Functional Characterization of cis-Elements Conferring Vascular Vein Expression of At4g34880 Amidase Family Protein Gene in Arabidopsis
文献类型： 外文期刊
作者： Wu, Xuelong 1 ; Huang, Ruizhi 2 ; Liu, Zhihong 2 ; Zhang, Guoping 1 ;
作者机构： 1.Zhejiang Univ, Dept Agron, Key Lab Crop Germplasm Resource, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Key Lab Plant Metab Engn Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2013 年 8 卷 7 期
页码：
收录情况： SCI
摘要： The expression of At4g34880 gene encoding amidase in Arabidopsis was characterized in this study. A promoter region of 1.5 kb on the upstream of the start codon of the gene (referred as AmidP) was fused with uidA (GUS) reporter gene, and transformed into Arabidopsis plant for determining its spatial expression. The results indicated that AmidP drived GUS expression in vascular system, predominately in leaves. Truncation analysis of AmidP demonstrated that VASCULAR VEIN ELEMENT (VVE) motif with a region of 176 bp sequence (-1500 to -1324) was necessary and sufficient to direct the vascular vein specific GUS expression in the transgenic plant. Tandem copy of VVE increased vascular system expression, and 5'- and 3'-deletions of VVE motif in combination with a truncated -65 CaMV 35S minimal promoter showed that 11bp cis-acting element, naming DOF2 domain, played an essential role for the vascular vein specific expression. Meanwhile, it was also observed that the other cis-acting elements among the VVE region are also associated with specificity or strength of GUS activities in vascular system.
- 相关文献
作者其他论文 更多>>
-
Rhizosphere inoculation of PGPR strain Bacillus cereus BC56 enhances salt tolerance of cucumber
作者：Song, Hanru;Wang, Huasen;Wu, Gengwei;Huang, Ruizhi;Gong, Xue;Wang, Hua
关键词：PGPR; B. cereus BC56; Salt tolerance; Cucumber; RNA-seq
-
Identification of the Key Transcription Factors Regulating the Expression of the Genes Associated with Barley Malt Quality during Malting
作者：Li, Mengdi;Zhang, Guoping;Ye, Lingzhen;Cai, Kangfeng;Zheng, Nannan;Zhang, Guoping;Ye, Lingzhen
关键词：barley (Hordeum vulgare L; ); hydrolyticenzyme; malt quality; transcriptome; WGCNA; PPI analysis
-
Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed Oil Accumulation and Chlorophyll Fluorescence Characteristics
作者：Huang, Ruizhi;Yang, Yong;Wu, Xuelong;Liu, Zhihong;He, Haiyan;Wu, Gengwei;Wang, Wengjia;Wang, Hua;Yu, Huasheng;Liu, Heqin
关键词：Brassica napus L.; heat stress; seed filling stage; oil accumulation; chlorophyll fluorescence characteristics
-
Transcriptomic and QTL Analysis of Seed Germination Vigor under Low Temperature in Weedy Rice WR04-6
作者：Wang, Wenjia;Huang, Ruizhi;Wu, Gengwei;Zhu, Ying;Wang, Hua;Sun, Jian
关键词：weedy rice; seed germination; low temperature; RNA-seq; QTL
-
Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses
作者：Cai, Kangfeng;Yue, Wenhao;Xia, Xue;Wang, Junmei;Cai, Kangfeng;Yue, Wenhao;Wang, Junmei;Kuang, Liuhui;Xie, Shanggeng;Zhang, Guoping
关键词：calmodulin; calmodulin-like; barley; expression; pan-genome; abiotic stress
-
Identification and characterization of HAK/KUP/KT potassium transporter gene family in barley and their expression under abiotic stress
作者：Cai, Kangfeng;Wang, Junmei;Cai, Kangfeng;Zeng, Fanrong;Zhang, Guoping
关键词：HAK; KUP; KT; Gene family; Barley; Gene expression; Abiotic stress response
-
Screening of Worldwide Barley Collection for Drought Tolerance: The Assessment of Various Physiological Measures as the Selection Criteria
作者：Cai, Kangfeng;Chen, Xiaohui;Han, Zhigang;Zhang, Shuo;Li, Qi;Nazir, Muhammad Mudassir;Zhang, Guoping;Zeng, Fanrong;Wu, Xiaojian
关键词：barley; drought tolerance; selection criterion; water relation; osmotic adjustment
