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Subcutaneous or Oral Immunization of Mice with Lactococcus lactis Expressing F4 Fimbrial Adhesin FaeG

文献类型： 外文期刊

作者： Liu, Shujie 1 ; Li, Yongming 1 ; Xu, Ziwei 1 ; Wang, Yicheng 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： enterotoxigenic Escherichia coli;FaeG;immune response;Lactococcus lactis

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2013 年 75 卷 6 期

页码：

收录情况： SCI

摘要： Enterotoxigenic Escherichia coli (ETEC) is one of the most common causes of diarrhea in neonatal and postweaning piglets. Fimbrial adhesion of ETEC has been considered an important colonization factor with antigenicity. To safely and effectively deliver the F4 (K88) fimbrial adhesin FaeG to the immune system, we have previously constructed the secretory expression vector pNZ8112-faeG, and FaeG was produced in cytoplasmic form in Lactococcus lactis. In this work, BALB/c mice were immunized with recombinant L. lactis to further determine the immunogenicity of recombinant FaeG (rFaeG) via the subcutaneous or oral route. Subcutaneous immunization in mice with recombinant L. lactis induced a significant increase in the F4-specific serum IgG titer and the number of antibody-secreting cells (ASCs) in the spleen. Oral immunization of mice with recombinant L. lactis induced mucosal and systemic F4-specific immune responses and increased the number of ASCs in the spleen, mesenteric lymph nodes and Peyer's patches. High-dose (2.8 x 10(11) CFU) recombinant strains and adjuvant cholera toxin B subunit enhanced specific mucosal immune responses. The results suggest the feasibility of delivering rFaeG expressed in L. lactis to the immune system in order to induce an F4-specific immune response.

  • 相关文献

[1]Induction of specific immune responses in piglets by intramuscular immunization with fimbrial adhesin FaeG expressed in Lactococcus lactis. Liu, Shujie,Li, Yongming,Xu, Ziwei. 2013

[2]产肠毒素大肠杆菌K88黏附素蛋白亚基Fae G在L.lactis中的表达及其抗原性. 刘淑杰,李永明,徐子伟,王一成. 2009

[3]Recombinant Lactococcus lactis expressing porcine insulin-like growth factor I ameliorates DSS-induced colitis in mice. Liu, Shujie,Li, Yongming,Deng, Bo,Xu, Ziwei. 2016

[4]Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Li, GJ,Chen, WZ,Yan, WY,Zhao, K,Liu, MQ,Zhang, J,Fei, L,Xu, QX,Sheng, ZT,Lu, YG,Zheng, ZX.

[5]Immune responses elicited in mice with recombinant Lactococcus lactis expressing F4 fimbrial adhesin FaeG by oral immunization. Liu, Shujie,Li, Yongming,Xu, Ziwei,Wang, Yicheng,Liu, Shujie,Xu, Ziwei.

[6]Greater efficacy of the ECMS-oil adjuvant over other formulations on immune responses against Bordetella bronchiseptica in rabbits and the underlying mechanism. Xiao, Chenwen,Bao, Guolian,Liu, Yan,Wei, Qiang,Ji, Quanan,Liu, Yaping,Pan, Lijun.

[7]Enhancement of immune responses to infectious bursal disease vaccine by supplement of an extract made from Momordica cochinchinensis (Lour.) Spreng. seeds. Rajput, Z. I.,Xiao, C. W.,Hu, S. H.,Rajput, Z. I.,Xiao, C. W.,Habib, M.,Soomro, N. A..

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