Molecular cloning, expression and regulation analysis of the interleukin-6 (IL-6) gene in goose adipocytes
文献类型： 外文期刊
作者： Wang, F. 2 ; Tian, Y. 1 ; Li, G. 1 ; Chen, X. 1 ; Yuan, H. 1 ; Wang, D. 1 ; Li, J. 1 ; Shen, J. 1 ; Tao, Z. 1 ; Fu, Y.; Lu, L. 1 ;
作者机构： 1.Zhejiang Univ, Inst Anim Husb & Vet Sci, Zhejiang Acad Agr Sci, Hangzhou 310003, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zheji
期刊名称：BRITISH POULTRY SCIENCE （ 影响因子：2.095； 五年影响因子：2.266 ）
ISSN： 0007-1668
年卷期： 2012 年 53 卷 6 期
页码：
收录情况： SCI
摘要： 1. Interleukin-6 (IL-6) is a multifunctional cytokine involved in lipid metabolism in adipose tissue. The objective of the study was to identify and characterize the IL-6 gene in the goose. 2. A full-length coding sequence (CDS) of the goose (Anser anser) IL-6 gene was cloned that encoded a 234-amino acid peptide containing a 38-amino acid signal peptide, an IL-6/G-CSF/MGF family consensus pattern and four conserved -helices. The mature goose IL-6 showed 74% and 39% identities to that of chicken and human, respectively. 3. Quantitative real-time PCR analysis showed that the goose IL-6 was predominantly expressed in liver and was up-regulated in adipocytes by lipopolysaccharide (LPS) and oleic acid.
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