Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus
文献类型： 外文期刊
作者： Yun, Tao 1 ; Ni, Zheng 1 ; Hua, Jionggang 1 ; Ye, Weicheng 1 ; Chen, Liu 1 ; Zhang, Shuai 1 ; Zhang, Yan 1 ; Zhang, Cun 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China
2.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China
关键词： Duck Tembusu virus;Real-time RT-PCR;Minor-groove-binding probe;Diagnostic assay
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2012 年 181 卷 2 期
页码：
收录情况： SCI
摘要： The design and development of a 3'-conjugated minor-groove-binding (MGB) probe for a real-time RT-PCR assay allowing for the rapid, sensitive, and specific detection of duck Tembusu virus (DTMUV) RNA are described. This assay targeted the 3' terminal non-coding region (NCR) of the TMUV genome and detected 1 x 10(1) copies of RNA per reaction without cross-reaction with other duck pathogens. The linear range of detection was 2 x 10(1)-2 x 10(8) copies/mu l. The assay was rapid, requiring just over 2.0 h, including the nucleic acid extraction step. Therefore, this assay is an excellent tool for research routine diagnostic applications, and study of the epidemiology of TMUV infections among duck flocks. (C) 2012 Elsevier B.V. All rights reserved.
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