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Overexpression of AtHsp90.3 in Arabidopsis thaliana impairs plant tolerance to heavy metal stress

文献类型: 外文期刊

作者: Song, H. M. 1 ; Wang, H. Z. 1 ; Xu, X. B. 1 ;

作者机构: 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

关键词: antioxidants;arsenic;cadmium;heat shock proteins;malondialdehyde;phytochelatins

期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )

ISSN: 0006-3134

年卷期: 2012 年 56 卷 1 期

页码:

收录情况: SCI

摘要: The functions of cytosolic heat shock protein AtHsp90.3 in response to heavy metal stress were characterized by using expression of AtHsp90.3 gene in yeast and Arabidopsis thaliana. AtHsp90.3 supported the Saccharomyces cerevisiae Hsp90 knockout strain R0005 growth and maintaining cells membrane integrity under cadmium and arsenic stresses, which was compatible with the components of ScHsc82 machinery. However, constitutive overexpression of AtHsp90.3 in Arabidopsis impaired plant tolerance to Cd stress with lower germination rate and shorter root length, decreased contents of phytochelatins (PCs) and glutathione (GSH), inhibited activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and increased content of malondialdehyde (MDA). These results suggested that proper homeostasis of Hsp90 was critical for cellular response and/or tolerance to heavy metal stress in plants.

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