您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Isolation of a novel peptide from silkworm pupae protein components and interaction characteristics to angiotensin I-converting enzyme

文献类型： 外文期刊

作者： Wang, Wei 1 ; Wang, Nan 2 ; Zhou, Yu 1 ; Zhang, Yu 1 ; Xu, Lihong 1 ; Xu, Junfeng 1 ; Feng, Fengqin 3 ; He, Guoqing 3 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Shuren Univ, Dept Food Sci & Nutr, Hangzhou 310029, Zhejiang, Peoples R China

3.Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310029, Zhejiang, Peoples R China

关键词： ACE;Silkworm pupae protein;Hydrolysate characteristics;Inhibitory rate;Flexible docking

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）

ISSN： 1438-2377

年卷期： 2011 年 232 卷 1 期

页码：

收录情况： SCI

摘要： The hydrolysate of silkworm protein produced by acid protease (Asperqiius usamii NO. 537) contains angiotensin I-converting enzyme inhibitory activity. Four kinds of protein components were identified in silkworm pupae protein, albumin, globulin, prolamin and glutelin, and the albumin was in the highest quantity. The four component proteins were then further hydrolyzed by acid protease under the same conditions, and the hydrolysate of albumin was determined with the highest ACE inhibiting effect, followed by globulin. Albumin could be easily hydrolyzed under the aqueous conditions, and the overall albumin protein content was higher than the other three protein components. The peptide sequence "APPPKK'' was determined by LC-MS/MS separation and X!Tandem software identification. The peptide inhibitory activity was 0.047 mg/mL in IC50. The peptide was bonded to Asp(415), Asp(453), Thr(282), His(353), Glu(162) in hydrogen bond to ACE active pocket by flexible docking calculations. This study indicates that silkworm pupae protein may be a suitable candidate to explore functional foods with anti-hypertension bioactivity.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台