您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of AFLP fragments linked to seedlessness in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers

文献类型： 外文期刊

作者： Xiao JinPing 1 ; Chen LiGeng 1 ; Xie Ming 2 ; Liu HaiLin 1 ; Ye WeiQi 3 ;

作者机构： 1.Zhejiang Univ, Lab Fruit Mol Physiol & Biotechnol, State Agr Minist Lab Hort Plant Growth Dev & Biot, Hangzhou 310029, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

3.Lishui Inst Agr Sci, Lishui 323000, Peoples R China

关键词： Seedlessness;AFLP;Ponkan mandarin;SCAR

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2009 年 121 卷 4 期

页码：

收录情况： SCI

摘要： Seedlessness is a desirable trait ill Citrus and has been an important breeding objective. In this Study, we employed the amplified fragment length polymorphism (AFLP) technique to find molecular markers linked to the seedless trait in the Ponkan mandarin (Citrus reticulata Blanco). After screening with 72 primer pairs, 5 AFLP markets were identified that putatively correlated with the target trait. Their association was also tested by analyzing the AFLP profile from pooled individual accessions. The five fragments were cloned and sequenced, and BLAST searches showed that four of the markers had high homology to functional genes, providing some promising information that may aid in understanding the molecular mechanism of seedlessness in citrus. Based on the sequence information, eight specific primers were designed and eventually fragments AFLP-2 and AFLP-5 were successfully converted into sequence characterized amplified region (SCAR) markers. Thus, the markers detected could be useful for accelerating citrus breeding programmes by enabling early screening for seedlessness mutations using marker-assisted selection. (c) 2009 Elsevier B.V. All rights reserved.

  • 相关文献

[1]番茄抗黄化曲叶病毒病基因的AFLP分子标记. 姚金晓,杨悦俭,叶青静,王荣青,阮美颖,周国治,姚祝平. 2010

[2]棉铃虫AFLP技术体系的建立. 牛宝龙,翁宏飚,孟智启. 2006

[3]Genetic evidence of local adaption and long distance migration in Blumeria graminis f. sp hordei populations from China. Zhu, Jinghuan,Shang, Yi,Hua, Wei,Wang, Junmei,Jia, Qiaojun,Yang, Jianming,Zhou, Yijun,Liu, Mengdao.

[4]Genetic variations within a collection of anthuriums unraveled by morphological traits and AFLP markers. Ge, Yaying,Zhang, Fei,Shen, Xiaolan,Yu, Yongming,Pan, Xiaoyun,Liu, Xiaojing,Liu, Jianxin,Pan, Gangmin,Tian, Danqing.

[5]Genetic relatedness among Aechmea species and hybrids inferred from AFLP markers and pedigree data. Zhang, Fei,Wang, Weiyong,Ge, Yaying,Shen, Xiaolan,Tian, Danqing,Liu, Jianxin,Liu, Xiaojing,Yu, Xinyin,Zhang, Zhi.

[6]基于AFLP的家蚕性连锁平衡致死系Z染色体连锁分子标记筛选. 王海龙,刘岩,牛宝龙,轩楠,孟智启. 2010

[7]全国7省本地早橘基因组间相关性的AFLP分析. 付春华,陈方永,邓秀新. 2006

[8]几个番茄品种AFLP指纹图谱的建立. 姚金晓,杨悦俭,叶青静,王荣青,周国治,阮美颖. 2009

[9]AFLP分子标记技术在浙蒲2号种子纯度快速鉴定中的应用. 王玲平,戴丹丽,吴晓花,汪宝根,李国景. 2008

[10]番茄抗青枯病种质资源遗传多样性的AFLP分析. 周国治,李志邈,杨悦俭,邢娟,王荣青. 2009

[11]长豇豆AFLP技术体系的构建与优化. 刘永华,李国景,吴晓花,汪宝根,鲁忠富,朱祝军. 2007

[12]应用AFLP方法研究中国大豆的遗传多样性(英文). 朱申龙,MortiML,RaoR. 1998

[13]番茄种质遗传多样性的AFLP分析. 姚祝平,叶青静,杨悦俭,王荣青,阮美颖,周国治. 2010

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台