您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cloning, sequencing and expression of a novel xylanase cDNA from a newly isolated Aspergillus awamori in Pichia pastoris

文献类型： 外文期刊

作者： Yang, Yinan 2 ; Shan, Kuixian 3 ; Ping, Lifeng 1 ; Lu, Jingmei 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

2.NE Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China

3.Jilin Agr Univ, Coll Hort, Changchun 130118, Peoples R China

关键词： Aspergillus awamori;cDNA;cloning;xylanase;eukaryotic expression;purification and characterization

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2008 年 7 卷 23 期

页码：

收录情况： SCI

摘要： A strain SH 2016, capable of producing xylanase, was isolated and identified as Aspergillus awamori, based on its physiological and biochemical characteristics as well as its ITS rDNA gene sequence analysis. A xylanase gene of 591 bp was cloned from this newly isolated A. awamori and the ORF sequence predicted a protein of 196 amino acids with a molecular mass about 21 kDa. An expression plasmid carrying the gene under the control of the methanol regulated alcohol oxidase gene (AOX1) promotor was introduced into Pichia pastoris, and xylanase gene was successfully expressed into the medium using methanol as inducer. Xylanase with 6 his tags was purified using Ni2+-NTA column. The characteristics of purified xylanase were investigated.

  • 相关文献

[1]Production of quercetin, kaempferol and their glycosidic derivatives from the aqueous-organic extracted residue of litchi pericarp with Aspergillus awamori. Lin, Sen,Zhu, Qinqin,Wen, Lingrong,Yang, Bao,Jiang, Guoxiang,Jiang, Yueming,Gao, Haiyan,Chen, Feng,Lin, Sen,Zhu, Qinqin,Jiang, Guoxiang.

[2]草莓花青苷分子调控机理的初步研究. 苗立祥. 2016

[3]应用RACE技术扩增鸭A-FABP基因及序列分析. 田勇,张聪聪,张红艳,李进军,卢立志. 2012

[4]Sequence analysis of a putative goose RIG-I gene. Li, Guoqin,Li, Jinjun,Tian, Yong,Wang, Deqian,Shen, Junda,Tao, Zhengrong,Xu, Jian,Lu, Lizhi. 2012

[5]Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase. Li, Yin,Liu, Zhiqiang,Liu, Zhiqiang,Li, Geng,Cui, Fengjie,Ping, Lifeng,Qiu, Chongyan,Yan, Lijiao,Yan, Lijiao. 2009

[6]Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19. Li, Yin,Cui, Fengjie,Liu, Zhiqiang,Ping, Lifeng,Xue, Yaping,Liu, Zhiqiang,Zhao, Hui,Ping, Lifeng,Yang, Yinan,Yan, Lijiao. 2009

[7]Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition. Xu, Yao-Xing,Li, Yan-li,Xu, Shao-chun,Liu, Yong,Wang, Xin,Tang, Jiang-wu. 2008

[8]Cloning, expression, and characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans YIM 90462(T). Zhang, Feng,Chen, Jiu-Jiu,Ren, Wan-Zeng,Zhi, Xiao-Yang,Tang, Shu-Kun,Li, Wen-Jun,Zhang, Feng,Chen, Jiu-Jiu,Ren, Wan-Zeng,Zhi, Xiao-Yang,Tang, Shu-Kun,Li, Wen-Jun,Chen, Jiu-Jiu,Lin, Lian-Bing,Zhou, Yu,Li, Wen-Jun.

[9]Cloning and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus of the ZJ Strain. Hao, Baocheng,Niu, Dengyuan,Xing, Xiaoyong,Xiang, Haitao,Wen, Fengqin,Fu, Xiaoping,Liang, Jianping,Hu, Yonghao,Niu, Dengyuan,Liang, Jianping. 2014

[10]Cloning, sequencing, and expression of a novel epoxide hydrolase gene from Rhodococcus opacus in Escherichia coli and characterization of enzyme. Liu, Zhiqiang,Li, Yin,Xu, Yingying,Ping, Lifeng,Zheng, Yuguo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台