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Mapping and expression analysis of chicken NDRG1 and NDRG3 genes

文献类型： 外文期刊

作者： Tian, Yong 1 ; Xu, Minlin 3 ; Fu, Yan 2 ; Yuan, Aiping 1 ; Wang, Deqian 1 ; Li, Guoqin 1 ; Liu, Gentao 3 ; Lu, Lizhi 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Lab Anim Genet, Hangzhou 310029, Peoples R China

3.Cedars Sinai Med Ctr, Maxine Dunitz Neurosurg Inst, Los Angeles, CA 90048 USA

4.Univ Calif Los Angeles, David Geffen Sch Med, Cedars Sinai Med Ctr, Div Hematol & Oncol, Los Angeles, CA 90048 USA

关键词： RH panel;mapping;genetic marker;NDRG1 gene;NDRG3 gene;chicken

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）

ISSN： 0006-2928

年卷期： 2008 年 46 卷 9-10 期

页码：

收录情况： SCI

摘要： N-myc downstream-regulated genes 1 and 3 (NDRG1 and NDRG3) are members of the alpha/beta hydrolase superfamily. Phylogenetic analysis of the family demonstrated that human NDRG1 and 3 belong to a subfamily. The mapping and gene expression patterns of these genes represent one step toward further investigation into their possible roles in the chicken (Gallus gallus). To map these genes in the chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. Primers were designed according to the published human sequences for amplification of those two genes. We compared the corresponding human mRNA sequences with the predicted coding sequences of the chicken NDRG1 and 3 genes and found that the assembled contigs shared a high percentage of similarity with the human genes. PCR of samples from ChickRH6 revealed that the locations of NDRG1 and 3 are linked to the markers MYC (58 cRs away, LOD score 4.52) and SEQ0265 (10 cRs away, LOD score 17.81), respectively. This result adds two new markers to the chicken RH map, and it reinforces that the RH technique is indeed a powerful tool for mapping genes due to its rapidity, precision, convenience, and reproducibility. In addition, we detected the gene expression and distribution of chicken NDRG1 and 3 in seven tissues, including heart, liver, spleen, lung, muscle, brain, and thymus, by RT-PCR, and found that NDRG1 is relatively ubiquitously expressed in all the tested tissues and highly expressed in heart and liver, whereas NDRG3 is high in heart, muscle, and brain.

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