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Detection and characterisation of a second potyvirus from Thunberg fritillary in China

文献类型: 外文期刊

作者: Chen, J 1 ; Zheng, HY 2 ; Shi, YH 2 ; Adams, MJ 2 ; Wei, CB 3 ; Lin, L; Chen, JP;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China; Zhejiang Univ, Coll Life Sci, Hangzhou 310027, Peoples R China; Rothamsted Res, Plant Pathogen Interact Div, Wheat Pathogenesis Programme, Harpenden, Herts, England; Wanxi Coll, Dept Biol, Liuan, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

ISSN: 0304-8608

年卷期: 2006 年 151 卷 3 期

页码:

收录情况: SCI

摘要: Plants of Thunberg fritillary (Fritillaria thunbergii Miq.) from Zhejiang Province, were found to be co-infected with two distinct potyviruses. One was an isolate of the recently reported Thunberg fritillary mosaic virus (TFMV; Wei et al., (2005) Arch Virol 150: 1271-1280), while the other was a distinct virus that did not react with TFMV antiserum nor with antisera to 17 other potyviruses, except for a weak reaction with antibodies produced to soybean mosaic virus (SMV) Pinellia strain. Both viruses could be transmitted mechanically to their original host but not to any of a range of commonly used indicator plants. No local lesion host was identified that would enable the viruses to be propagated independently. The complete sequences of both viruses were determined; that of the new virus (9656 nt) had the typical genome organisation and recognised sequence motifs of a potyvirus, encoding a putative polyprotein of 351 kDa. Phylogenetic analysis, sequence comparisons, and the pattern of polyprotein cleavage sites all indicated that it was a member of the Bean common mosaic virus subgroup. The most closely related species are Soybean mosaic virus and Wisteria vein mosaic virus, with 68-69% amino acid identity between their polyproteins. This is sufficiently different for the new virus to be regarded as a distinct species, which we have tentatively named Fritillary virus Y.

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