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Proteomic studies of lipopolysaccharide-induced polypeptides in the silkworm, Bombyx mori

文献类型: 外文期刊

作者: Wang, YQ 1 ; Zhang, PB 2 ; Fujii, H 2 ; Banno, Y 2 ; Yamamoto, K 2 ; Aso, Y 2 ;

作者机构: 1.Kyushu Univ, Fac Agr, Labs Insect Genet Resources, Fukuoka 8128581, Japan

2.Kyushu Univ, Fac Agr, Labs Insect Genet Resources, Fukuoka 8128581, Japan; Zhejiang Acad Agr Sci, Hangzhou 310021, Peoples R China

关键词: proteomics;silkworm;peptide mass fingerprinting;lipopolysaccharide;attacin

期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )

ISSN: 0916-8451

年卷期: 2004 年 68 卷 8 期

页码:

收录情况: SCI

摘要: Silkworm larvae at the 5th instar were injected with lipopolysaccharide from Escherichia coli and inducible polypeptides were examined within a pI range of 3-10 and a size range of 14-97 kDa by proteomics, including peptide mass fingerprinting. No polypeptides were induced in the midgut. FB1 and H1-4 polypeptides were significantly induced in fat body and hemolymph, respectively. FB1 and HI were estimated to be antitrypsin and serpin-2 proteinase inhibitors respectively. H2 and H3 were novel polypeptides. H4 was estimated to be attacin antibacterial polypeptide with high coverage of sequence. The amounts of all the induced polypeptides decreased at 48 h after the injection.

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