文献类型： 外文期刊

作者： Kobayashi, S ¹ ; Ishimaru, M ² ; Ding, CK ³ ; Yakushiji, H ⁴ ; Goto, N ⁵ ;

作者机构： 1.Natl Inst Fruit Tree Sci, Persimmon & Grape Res Ctr, Akitsu, Hiroshima 7292494, Japan

2.Univ Osaka Prefecture, Grad Sch Agr & Biol Sci, Sakai, Osaka 5998531, Japan

3.Natl Inst Fruit Tree Sci, Dept Breeding, Tsukuba, Ibaraki 3058605, Japan

4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China

5.Natl Res Inst Brewing, Hiroshima 7390046, Japan

关键词： anthocyanin;grape;UDP-glucose : flavonoid 3-O-glucosyltransferase;in vitro recombination

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2001 年 160 卷 3 期

页码：

收录情况： SCI

摘要： The expression of the UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene has been shown to be critical for anthocyanin biosynthesis in the grape berry. Using white cultivars and bud sports with red skin, we examined the expression of seven anthocyanin biosynthetic genes including the UFGT gene and compared the coding/promoter sequences of the UFGT gene. Northern blot analysis showed that the seven anthocyanin biosynthetic genes were expressed coordinately at higher levels in the red-skin sports than in the while-skin progenitors of the sports. It was especially notable that UFGT gene expression was detected only in the red-skin sports and Kyoho. However, there were no differences in either coding or promoter sequences between Italia (Vitis vinifera) and its red-skin sport Ruby Okuyama, or between Muscat of Alexandria (V. vinifera) and the red-skin sport Flame Muscat. From these findings, the phenotypic change from white to red in the sports is thought to be the result of a mutation in a regulatory gene controlling the expression of UFGT. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.