文献类型: 外文期刊
作者: Luo, ZL 1 ; Chen, XY 2 ; Gao, DM 2 ; Fang, RX 2 ;
作者机构: 1.Chinese Acad Sci, Inst Microbiol, Lab Plant Biotechnol, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, Lab Plant Biotechnol, Beijing 100080, Peoples R China; Zhejiang Acad Agr Sci, Inst Virol, Hangzhou 310021, Peoples R China
关键词: RYSV;rhabdovirus;gene 4;nucleotide sequence;matrix protein
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN: 0920-8569
年卷期: 1998 年 16 卷 3 期
页码:
收录情况: SCI
摘要: The complete nucleotide sequence of the gene 4 of rice yellow stunt rhabdovirus (RYSV) was determined from cDNAs corresponding to the viral genomic RNA. Gene 4 is 913 nucleotides (nt) long, comprising a 17-nt untranslated 5' region, a 786-nt open reading frame encoding a polypeptide with a molecular mass of 29,125 Da, and a 110-nt untranslated 3' region. Western blot analysis of the RYSV proteins using the antiserum raised against the protein expressed from the cloned gene in Escherichia coli indicates that gene 4 encodes the M protein of RYSV. Comparisons of the deduced amino acid sequence of the M protein of RYSV with those of other rhabdoviruses revealed no significant homologies. However, it shared a similar basic property and a similar distribution of charges with the other rhabdovirus matrix proteins and showed a relatively closer relationship to the sonchus yellow net virus (SYNV) M1 protein.
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