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A GENETIC-ANALYSIS OF A TOMATO (LYCOPERSICON-ESCULENTUM) GENOTYPE WITH A HIGH-FREQUENCY OF TWIN SPOTS

文献类型: 外文期刊

作者: ZHU, D 1 ; SCHOENMAKERS, HCH 2 ; WOLTERS, AMA 2 ; KOORNNEEF, M 2 ;

作者机构: 1.AGR UNIV WAGENINGEN,DEPT GENET,6703 HA WAGENINGEN,NETHERLANDS

2.AGR UNIV WAGENINGEN,DEPT GENET,6703 HA WAGENINGEN,NETHERLANDS; ZHEJIANG ACAD AGR SCI,CROP RES INST,HANGZHOU 310021,PEOPLES R CHINA

关键词: GENETIC INSTABILITY;SOMACLONAL VARIATION;TWIN SPOTS;LYCOPERSICON ESCULENTUM;TOMATO

期刊名称:THEORETICAL AND APPLIED GENETICS ( 影响因子:5.699; 五年影响因子:5.565 )

ISSN: 0040-5752

年卷期: 1995 年 91 卷 6-7 期

页码:

收录情况: SCI

摘要: Among pale-green tomato plants heterozygous for the xanthophyllic 2 (xa-2) mutation that were transformed with a T-DNA harbouring the NPTII and GUS gene, a plant with a high frequency of green/white twin spots was found. The genetic analysis of this plant indicated that the occurrence of these twin spots was caused by a genetic defect located at the distal end of chromosome 10S, where xa-2 also is located. The genetic analysis of green plants regenerated from leaf explants of this twin-spot plant revealed that the green sectors derive from non-disjunction of the xa-2(+) allele. In an analysis of mitotic chromosome behaviour bridges were observed in approximately 5% of the anaphases, providing arguments that a breakage-fusion-bridge-cycle caused by a tissue culture-induced genomic instability is the most likely cause of this aberrant behaviour of chromosome 10.

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