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Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response

文献类型： 外文期刊

作者： Liu, Linya 1 ; Huang, Yacheng 1 ; Huang, Xiaolong 3 ; Yang, Jianghua 4 ; Wu, Wenqiang 1 ; Xu, Yun 1 ; Cong, Ziwen 1 ; Xie 1 ;

作者机构： 1.Hainan Univ, Inst Trop Agr & Forestry, Haikou 570228, Hainan, Peoples R China

2.Liupanshui Normal Univ, Sch Biol Sci & Technol, Liupanshui 553001, Peoples R China

3.Hainan Univ, Inst Trop Agr & Forestry, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China

4.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Peoples R China

关键词： dioscorin;cloning and expression;tuber development;promoter;Dioscorea alata L

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2017 年 18 卷 7 期

页码：

收录情况： SCI

摘要： Dioscorin is one of the major soluble proteins in yam tubers. Unlike other well-known plant storage proteins, such as patatin and sporamin, dioscorin is argued for its function as storage proteins, and the molecular mechanisms underlying its expressional complexity are little understood. In this study, we isolated five dioscorin genes from Dioscorea alata L., comprising three class A (Da-dio1, -3 and -4) and two class B (Da-dio2 and -5) isoforms. Expressions of all dioscorin genes gradually decreased in mother tubers during yam sprouting and regrowth. On the other hand, all dioscorin genes accumulated transcripts progressively with tuber development in new tubers, with Da-dio5 being the most prominent isoform. In yam leaves, the expressions of Da-dio5 were up-regulated by the treatments of five phytohormones (gibberellic acid, salicylic acid, indole-3-acetic acid, abscisic acid, and ethylene), and three abiotic stresses (high-temperature, low-temperature and drought). To further elucidate the regulatory mechanisms of Da-dio5 expressions, transgenic Arabidopsis plants harboring the Da-dio5 promoter-beta-glucuronidase (GUS) fusion were generated. GUS staining showed that expressions of the Da-dio5 promoter were detected mainly in the shoot apical meristem (SAM) and hypocotyls, and enhanced by the treatments of the five hormones, and the three abiotic stresses mentioned above. These results suggest diverse roles of Da-dio5 in yam sprouting, regrowth, and tuberization, as well as in response to enviromental cues.

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