Reduction of reactive red 241 by oxygen insensitive azoreductase purified from a novel strain Staphylococcus KU898286
文献类型： 外文期刊
作者： Nisar, Numrah 1 ; Aleem, Amber 1 ; Saleem, Faiza 2 ; Aslam, Fakhra 1 ; Shahid, Ammara 1 ; Chaudhry, Hina 1 ; Malik, Ka 1 ;
作者机构： 1.Lahore Coll Women Univ Lahore, Dept Environm Sci, Lahore, Pakistan
2.Lahore Coll Women Univ Lahore, Dept Biotechnol, Lahore, Pakistan
3.Lahore Coll Women Univ Lahore, Dept ORIC, Lahore, Pakistan
4.Univ Sebha, Fac Sci, Sebha, Libya
5.Chinese Acad Trop Agr Sci, Hainan Key Lab Trop Oil Crops Biol, Coconut Res Inst, Wenchang, Hainan, Peoples R China
6.Abdul Wali Khan Univ Mardan, Dept Agr, Mardan, Pakistan
7.Univ Faisalabad, Inst Hort Sci, Faisalabad, Pakistan
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2017 年 12 卷 5 期
页码：
收录情况： SCI
摘要： An oxygen insensitive azoreductase was purified from a novel bacterial strain (Staphylococcus sp. KU898286) that was isolated from an abandoned site of the textile waste discharge unit. The isolated enzyme had efficiently cleaved the azo-bonds through reductive transformation under aerobic conditions. Initial phenotypic characterization and final construction of phylogenetic tree on the basis of 16s rDNA demonstrated 99% resemblance of the isolate to Staphylococcus aureus. The purified azoreductase was found to have a broad spectrum activity that reduced RR241 at a concentration of 50mg/L with pH between 6-8 and 30 degrees C temperature). Besides, the reactive red 241 (RR241) was reduced at extracellular level as well as NADH dependent intracellular level. Complete reduction/decolourization of RR241 were achieved after 18 hrs of exposure. The final degradation product observed to be 2-nephthol was purified by High Pressure Liquid Chromatography (HPLC) and the molecular mass was computed by Gas Chromatography-Mass spectroscopy (GC-MS). The study revealed a cost effective and eco-friendly approach to degrade the toxic dyes into less toxic products by Staphylococcus sp. KU898286.
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